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In silico PCR verification and simplex real-time PCR detection of methicillin-resistant Staphylococcus aureus (MRSA) from east coast Malaysian clinical isolates

The aims of this study were to validate the primers developed for molecular-based detection and identification of Malaysian clinical isolates of methicilin-resistance Staphylococcus au reus (MRSA) using in-silico Polymerase Chain Reaction (PCR) and real-time PCR SYBR with Green I. Rapid molecular di...

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Main Author: Zarizal, Suhaili
Format: Article
Language:English
Published: 2013
Subjects:
S Agriculture (General)
Online Access:http://eprints.unisza.edu.my/2672/1/FH02-FBIM-17-10891.jpg
http://eprints.unisza.edu.my/2672/
https://www.scopus.com/record/display.uri?eid=2-s2.0-8488424
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spelling my-unisza-ir.26722021-08-21T08:09:51Z http://eprints.unisza.edu.my/2672/ In silico PCR verification and simplex real-time PCR detection of methicillin-resistant Staphylococcus aureus (MRSA) from east coast Malaysian clinical isolates Zarizal, Suhaili S Agriculture (General) The aims of this study were to validate the primers developed for molecular-based detection and identification of Malaysian clinical isolates of methicilin-resistance Staphylococcus au reus (MRSA) using in-silico Polymerase Chain Reaction (PCR) and real-time PCR SYBR with Green I. Rapid molecular diagnostic and risk assessment of the MRSA are possible by real-time PCR SYBR Green I. However, validation of such primers for real-life samples is expensive and time consuming. Hence, development and verification of real-time PCR primers by in-silico PCR can be the first step I' • I • Igo .os es - • II- • • • 'I . • ' • 8, 2013-01 Article NonPeerReviewed image en http://eprints.unisza.edu.my/2672/1/FH02-FBIM-17-10891.jpg Zarizal, Suhaili (2013) In silico PCR verification and simplex real-time PCR detection of methicillin-resistant Staphylococcus aureus (MRSA) from east coast Malaysian clinical isolates. Walailak Journal of Science and Technology, 10 (3). pp. 237-246. ISSN 16863933 https://www.scopus.com/record/display.uri?eid=2-s2.0-8488424
institution Universiti Sultan Zainal Abidin
building UNISZA Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Sultan Zainal Abidin
content_source UNISZA Institutional Repository
url_provider https://eprints.unisza.edu.my/
language English
topic S Agriculture (General)
spellingShingle S Agriculture (General)
Zarizal, Suhaili
In silico PCR verification and simplex real-time PCR detection of methicillin-resistant Staphylococcus aureus (MRSA) from east coast Malaysian clinical isolates
description The aims of this study were to validate the primers developed for molecular-based detection and identification of Malaysian clinical isolates of methicilin-resistance Staphylococcus au reus (MRSA) using in-silico Polymerase Chain Reaction (PCR) and real-time PCR SYBR with Green I. Rapid molecular diagnostic and risk assessment of the MRSA are possible by real-time PCR SYBR Green I. However, validation of such primers for real-life samples is expensive and time consuming. Hence, development and verification of real-time PCR primers by in-silico PCR can be the first step I' • I • Igo .os es - • II- • • • 'I . • ' • 8,
format Article
author Zarizal, Suhaili
author_facet Zarizal, Suhaili
author_sort Zarizal, Suhaili
title In silico PCR verification and simplex real-time PCR detection of methicillin-resistant Staphylococcus aureus (MRSA) from east coast Malaysian clinical isolates
title_short In silico PCR verification and simplex real-time PCR detection of methicillin-resistant Staphylococcus aureus (MRSA) from east coast Malaysian clinical isolates
title_full In silico PCR verification and simplex real-time PCR detection of methicillin-resistant Staphylococcus aureus (MRSA) from east coast Malaysian clinical isolates
title_fullStr In silico PCR verification and simplex real-time PCR detection of methicillin-resistant Staphylococcus aureus (MRSA) from east coast Malaysian clinical isolates
title_full_unstemmed In silico PCR verification and simplex real-time PCR detection of methicillin-resistant Staphylococcus aureus (MRSA) from east coast Malaysian clinical isolates
title_sort in silico pcr verification and simplex real-time pcr detection of methicillin-resistant staphylococcus aureus (mrsa) from east coast malaysian clinical isolates
publishDate 2013
url http://eprints.unisza.edu.my/2672/1/FH02-FBIM-17-10891.jpg
http://eprints.unisza.edu.my/2672/
https://www.scopus.com/record/display.uri?eid=2-s2.0-8488424
_version_ 1709673167130198016
score 13.211869

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