Quantitative PCR analysis of genes expressed during biofilm development of methicillin resistant Staphylococcus aureus (MRSA)

• Main Authors: Atshan, Salman Sahab, Shamsudin, Mariana Nor, Karunanidhi, Arunkumar, Belkum, Alex van, Lung, Leslie Than Thian, Sekawi, Zamberi, Nathan, Jayakayatri Jeevajothi, Ling, King Hwa, Seng, Johnson Shueh Chong, Alreshidi, Mateq Ali, Abduljaleel, Salwa A., Awang Hamat, Rukman
• Format: Article
• Published: Elsevier 2013
• Online Access: http://psasir.upm.edu.my/id/eprint/29540/

Staphylococcus aureus biofilm associated infections remains a major clinical concern in patients with indwelling devices. Quantitative real-time PCR (qPCR) can be used to investigate the pathogenic role of such biofilms. We describe qPCRs for 12 adhesion and biofilm-related genes of four S. aureus i...