Effect of Non-Hydrogen Peroxide on Antibacterial Activities of Meliponini and Apis Honeys against Staphylococcus aureus

• Main Authors: Mohd Hilmi, Abu Bakar, Fatimah, Ibrahim Jibril, Manivannan, Lavaniya
• Format: Conference or Workshop Item
• Language: English
• Published: 2017
• Subjects: QH301 Biology
• Online Access: http://eprints.unisza.edu.my/1195/1/FH03-FSK-18-12122.pdf; http://eprints.unisza.edu.my/1195/

Introduction: Honey is one of the greatest natural sources, enables to eliminate deterioration from infectious microorganisms as a result of non-hydrogen peroxide compound. The study aims to explore the effect of non-hydrogen peroxide derived from Meliponini and Apis honeys against Staphylococcus aureus. Materials and Methods: Meliponini honey from stingless bee and Apis honey from non-stingless bee were used in the analysis of agar well diffusion assay that contained Staphylococcus aureus grown cultures. An amplex red hydrogen peroxide kit was used to identify the presence of hydrogen peroxide in the honey. Meanwhile, non-hydrogen peroxide activity was confirmed after catalase treatment has shown negative results. Results: Hydrogen peroxide was absent in all Meliponini honeys however, it present in Apis honey. Conclusion: Antibacterial activity of Meliponini honey are solely effected by non-hydrogen peroxide. Meanwhile, diverse effect between hydrogen peroxide and non-hydrogen peroxide on antibacterial activity in Apis honey.