The effects of 2,4-Dichlorophenoxyacetic Acid on the induction of callus from cotyledon and hypocotyl explants of Butterfly Pea (Clitoria ternatea)
Clitoria ternatea (Butterfly pea) is a tropical medicinal and fodder legume from the Fabaceae family possessing various beneficial phytochemical compounds linked to the mammalian neuroprotective mechanism. Callus and cell suspension cultures are excellent alternatives for harnessing secondary metabo...
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Penerbit Universiti Kebangsaan Malaysia
2023
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my-ukm.journal.224542023-11-08T08:28:01Z http://journalarticle.ukm.my/22454/ The effects of 2,4-Dichlorophenoxyacetic Acid on the induction of callus from cotyledon and hypocotyl explants of Butterfly Pea (Clitoria ternatea) Siew, Chin Teoh Sreeramanan Subramaniam, Bee, Lynn Chew Clitoria ternatea (Butterfly pea) is a tropical medicinal and fodder legume from the Fabaceae family possessing various beneficial phytochemical compounds linked to the mammalian neuroprotective mechanism. Callus and cell suspension cultures are excellent alternatives for harnessing secondary metabolites from medicinal plants. The current study aims to induce callus from cotyledon and hypocotyl explants of C. ternatea for the establishment of cell suspension cultures. Cotyledon and hypocotyl explants from two-weeks-old seedlings were subjected to half-strength MS medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) at different concentrations (0.5 mg/L to 2.5 mg/L) and callus scoring and morphology were assessed at week 8 of culture. Results revealed that the treatment of 0.5 mg/L 2,4-D resulted in the highest percentage of callus induction (100%) and the highest callus scoring for both cotyledon and hypocotyl explants with friable callus morphology. Cotyledon explants exhibited a higher callus scoring with a relative value of 3.03 ± 0.20 compared to hypocotyl explants at 1.80 ± 0.12. This study thereby provides a basis for future studies on callus induction studies and the establishment of cell suspension cultures of C. ternatea for the production of valuable secondary metabolites linked to the memory enhancing properties of the plant. Penerbit Universiti Kebangsaan Malaysia 2023 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/22454/1/JM%205.pdf Siew, Chin Teoh and Sreeramanan Subramaniam, and Bee, Lynn Chew (2023) The effects of 2,4-Dichlorophenoxyacetic Acid on the induction of callus from cotyledon and hypocotyl explants of Butterfly Pea (Clitoria ternatea). Malaysian Applied Biology, 52 (1). pp. 61-72. ISSN 0126-8643 https://jms.mabjournal.com/index.php/mab/index |
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Clitoria ternatea (Butterfly pea) is a tropical medicinal and fodder legume from the Fabaceae family possessing various beneficial phytochemical compounds linked to the mammalian neuroprotective mechanism. Callus and cell suspension cultures are excellent alternatives for harnessing secondary metabolites from medicinal plants. The current study aims to induce callus from cotyledon and hypocotyl explants of C. ternatea for the establishment of cell suspension cultures. Cotyledon and hypocotyl explants from two-weeks-old seedlings were subjected to half-strength MS medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) at different concentrations (0.5 mg/L to 2.5 mg/L) and callus scoring and morphology were assessed at week 8 of culture. Results revealed that the treatment of 0.5 mg/L 2,4-D resulted in the highest percentage of callus induction (100%) and the highest callus scoring for both cotyledon and hypocotyl explants with friable callus morphology. Cotyledon explants exhibited a higher callus scoring with a relative value of 3.03 ± 0.20 compared to hypocotyl explants at 1.80 ± 0.12. This study thereby provides a basis for future studies on callus induction studies and the establishment of cell suspension cultures of C. ternatea for the production of valuable secondary metabolites linked to the memory enhancing properties of the plant. |
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Siew, Chin Teoh Sreeramanan Subramaniam, Bee, Lynn Chew |
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Siew, Chin Teoh Sreeramanan Subramaniam, Bee, Lynn Chew The effects of 2,4-Dichlorophenoxyacetic Acid on the induction of callus from cotyledon and hypocotyl explants of Butterfly Pea (Clitoria ternatea) |
author_facet |
Siew, Chin Teoh Sreeramanan Subramaniam, Bee, Lynn Chew |
author_sort |
Siew, Chin Teoh |
title |
The effects of 2,4-Dichlorophenoxyacetic Acid on the induction of callus from cotyledon and hypocotyl explants of Butterfly Pea (Clitoria ternatea) |
title_short |
The effects of 2,4-Dichlorophenoxyacetic Acid on the induction of callus from cotyledon and hypocotyl explants of Butterfly Pea (Clitoria ternatea) |
title_full |
The effects of 2,4-Dichlorophenoxyacetic Acid on the induction of callus from cotyledon and hypocotyl explants of Butterfly Pea (Clitoria ternatea) |
title_fullStr |
The effects of 2,4-Dichlorophenoxyacetic Acid on the induction of callus from cotyledon and hypocotyl explants of Butterfly Pea (Clitoria ternatea) |
title_full_unstemmed |
The effects of 2,4-Dichlorophenoxyacetic Acid on the induction of callus from cotyledon and hypocotyl explants of Butterfly Pea (Clitoria ternatea) |
title_sort |
effects of 2,4-dichlorophenoxyacetic acid on the induction of callus from cotyledon and hypocotyl explants of butterfly pea (clitoria ternatea) |
publisher |
Penerbit Universiti Kebangsaan Malaysia |
publishDate |
2023 |
url |
http://journalarticle.ukm.my/22454/1/JM%205.pdf http://journalarticle.ukm.my/22454/ https://jms.mabjournal.com/index.php/mab/index |
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1783877703143784448 |
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13.160551 |