Cotyledon with hypocotyl segment as an explant for the production of transgenic citrullus vulgaris schrad (watermelon) mediated by agrobacterium tumefaciens

• Main Authors: Suratman, F., Huyop, Fahrul Zaman, Wagiran, A., Rahmat, Z., Ghazali, H., Parveez, G. K. A.
• Format: Article
• Published: Asian Network for Scientific Information 2010
• Subjects: QK Botany; T Technology (General)
• Online Access: http://eprints.utm.my/id/eprint/25088/; http://scialert.net/abstract/?doi=biotech.2010.106.118

In this study, we describe the successful production of transgenic watermelon using Agrobacterium tumefaciens after optimizing a couple of transformation parameters using cotyledon with hypocotyl segment from 5 day old in vitro seedlings as target tissues. It was found that the best condition for tr...