Extraction optimization, purification and immunostimulatory activity in vitro of polyphenols from apple (Malus domestica) peel
Apple peel polyphenols were extracted by ultrasound-assisted extraction (UAE) and conditions for the extraction of polyphenols were optimized by the response surface methodology. The optimized conditions determined as 32 min of extraction time, 29 °C of extraction temperature and ethanol concentra...
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my-ukm.journal.156192020-11-10T09:02:48Z http://journalarticle.ukm.my/15619/ Extraction optimization, purification and immunostimulatory activity in vitro of polyphenols from apple (Malus domestica) peel Riaz, Asad Yousaf, Khurram Mohamed Abdin, Jabbar, Saqib Muhammad Abid, Muhammad Hashim, Malik Apple peel polyphenols were extracted by ultrasound-assisted extraction (UAE) and conditions for the extraction of polyphenols were optimized by the response surface methodology. The optimized conditions determined as 32 min of extraction time, 29 °C of extraction temperature and ethanol concentration of 56% with a yield of 35.08 ± 0.26 mg gallic acid equivalent/g dry weight. Six resins were used for the purification and D101 resin showed the highest ratio of desorption for polyphenols and further applied for dynamic adsorption/desorption test. Catechin, procyandin B, querecitin-3-galactoside and querecitin xyloside were identified in purified polyphenols by HPLC-MS/MS. Finally, the immunostimulatory activity in vitro of purified polyphenols on RAW264.7 cell lines was evaluated. The apple peel polyphenols exhibited a dose-dependent effect (p < 0.05) on the strongest propagation of RAW264.7 cells. The results demonstrate that apple peel is a valuable source of polyphenols and could be used as a natural immunostimulating agent for application in functional foods. Penerbit Universiti Kebangsaan Malaysia Article PeerReviewed application/pdf en http://journalarticle.ukm.my/15619/1/8.pdf Riaz, Asad and Yousaf, Khurram and Mohamed Abdin, and Jabbar, Saqib and Muhammad Abid, and Muhammad Hashim, Malik Extraction optimization, purification and immunostimulatory activity in vitro of polyphenols from apple (Malus domestica) peel. Sains Malaysiana, 49 (7). pp. 1553-1566. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid49bil7_2020/KandunganJilid49Bil7_2020.html |
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Apple peel polyphenols were extracted by ultrasound-assisted extraction (UAE) and conditions for the extraction of
polyphenols were optimized by the response surface methodology. The optimized conditions determined as 32 min of
extraction time, 29 °C of extraction temperature and ethanol concentration of 56% with a yield of 35.08 ± 0.26 mg
gallic acid equivalent/g dry weight. Six resins were used for the purification and D101 resin showed the highest ratio
of desorption for polyphenols and further applied for dynamic adsorption/desorption test. Catechin, procyandin B,
querecitin-3-galactoside and querecitin xyloside were identified in purified polyphenols by HPLC-MS/MS. Finally, the
immunostimulatory activity in vitro of purified polyphenols on RAW264.7 cell lines was evaluated. The apple peel
polyphenols exhibited a dose-dependent effect (p < 0.05) on the strongest propagation of RAW264.7 cells. The results
demonstrate that apple peel is a valuable source of polyphenols and could be used as a natural immunostimulating
agent for application in functional foods. |
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Article |
author |
Riaz, Asad Yousaf, Khurram Mohamed Abdin, Jabbar, Saqib Muhammad Abid, Muhammad Hashim, Malik |
spellingShingle |
Riaz, Asad Yousaf, Khurram Mohamed Abdin, Jabbar, Saqib Muhammad Abid, Muhammad Hashim, Malik Extraction optimization, purification and immunostimulatory activity in vitro of polyphenols from apple (Malus domestica) peel |
author_facet |
Riaz, Asad Yousaf, Khurram Mohamed Abdin, Jabbar, Saqib Muhammad Abid, Muhammad Hashim, Malik |
author_sort |
Riaz, Asad |
title |
Extraction optimization, purification and immunostimulatory activity in vitro of polyphenols from apple (Malus domestica) peel |
title_short |
Extraction optimization, purification and immunostimulatory activity in vitro of polyphenols from apple (Malus domestica) peel |
title_full |
Extraction optimization, purification and immunostimulatory activity in vitro of polyphenols from apple (Malus domestica) peel |
title_fullStr |
Extraction optimization, purification and immunostimulatory activity in vitro of polyphenols from apple (Malus domestica) peel |
title_full_unstemmed |
Extraction optimization, purification and immunostimulatory activity in vitro of polyphenols from apple (Malus domestica) peel |
title_sort |
extraction optimization, purification and immunostimulatory activity in vitro of polyphenols from apple (malus domestica) peel |
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Penerbit Universiti Kebangsaan Malaysia |
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http://journalarticle.ukm.my/15619/1/8.pdf http://journalarticle.ukm.my/15619/ http://www.ukm.my/jsm/malay_journals/jilid49bil7_2020/KandunganJilid49Bil7_2020.html |
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