Extraction optimization, purification and immunostimulatory activity in vitro of polyphenols from apple (Malus domestica) peel
Apple peel polyphenols were extracted by ultrasound-assisted extraction (UAE) and conditions for the extraction of polyphenols were optimized by the response surface methodology. The optimized conditions determined as 32 min of extraction time, 29 °C of extraction temperature and ethanol concentra...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
|
| Online Access: | http://journalarticle.ukm.my/15619/1/8.pdf http://journalarticle.ukm.my/15619/ http://www.ukm.my/jsm/malay_journals/jilid49bil7_2020/KandunganJilid49Bil7_2020.html |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Apple peel polyphenols were extracted by ultrasound-assisted extraction (UAE) and conditions for the extraction of
polyphenols were optimized by the response surface methodology. The optimized conditions determined as 32 min of
extraction time, 29 °C of extraction temperature and ethanol concentration of 56% with a yield of 35.08 ± 0.26 mg
gallic acid equivalent/g dry weight. Six resins were used for the purification and D101 resin showed the highest ratio
of desorption for polyphenols and further applied for dynamic adsorption/desorption test. Catechin, procyandin B,
querecitin-3-galactoside and querecitin xyloside were identified in purified polyphenols by HPLC-MS/MS. Finally, the
immunostimulatory activity in vitro of purified polyphenols on RAW264.7 cell lines was evaluated. The apple peel
polyphenols exhibited a dose-dependent effect (p < 0.05) on the strongest propagation of RAW264.7 cells. The results
demonstrate that apple peel is a valuable source of polyphenols and could be used as a natural immunostimulating
agent for application in functional foods. |
|---|