Optimization of Nigella sativa oil-based ferulate ester synthesis using response surface methodology
Nigella sativa L. seed, also recognized as black cumin is a medicinal plant that possesses miraculous power of healing due to the abilities to cure various ailments. Owing to the oily properties of plant oil, the oil ester is more preferable exhibiting special features such as non-toxic, outstandi...
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Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
Penerbit Universiti Kebangsaan Malaysia
2019
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Online Access: | http://journalarticle.ukm.my/14662/1/48_02_04.pdf http://journalarticle.ukm.my/14662/ http://www.mabjournal.com/index.php?option=com_content&view=article&id=921&catid=59:current-view&Itemid=56 |
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Summary: | Nigella sativa L. seed, also recognized as black cumin is a medicinal plant that possesses miraculous power of healing due to
the abilities to cure various ailments. Owing to the oily properties of plant oil, the oil ester is more preferable exhibiting
special features such as non-toxic, outstanding moisturizing action and most importantly the absence of oily texture. In this
study, Nigella sativa oil-based ferulate ester was synthesized via transesterification of Nigella sativa oil (NSO) and ethyl
ferulate (EF) in the presence of immobilized lipase, Lipozyme RM IM from Rhizomucor miehei. A five-level-four-factor
central composite rotatable design (CCRD) from response surface methodology (RSM) was applied to study the influence of
synthesis parameters: lipase dosage (50-80 mg), temperature (40-60°C), substrate ratio of NSO:EF (2.5:1-5.5:1 w/w) and
time (3-7 hr) aiming for the maximum percentage yield of ester. The optimized synthesis conditions were lipase dosage of 67
mg, temperature of 56°C, substrate ratio (NSO:EF) of 4.4:1 w/w and time of 4 hr. The maximum production of ester obtained
was 49.87% which is acceptable with the predicted value of 48.82%. |
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