Enhanced large-scale production of recombinant phytase in E. coli DH5 α: Medium components optimization and thermodynamic studies

• Main Authors: Nuge, T., Hayyan, A., Elgharbawy, A.A.M., Salleh, H.M., Jun Yong, Y., Kamarudin, A.F., Hizaddin, H.F., Zuhanis Has-Yun Hashim, Y., Liu, X., Saleh, J., Ibrahim Daoud, J., Aljohani, A.S.M., Alhumaydhi, F.A., Zulkifli, M.Y., Roslan Mohd Nor, M., Al Abdulmonem, W.
• Format: Article
• Published: 2023
• Online Access: http://scholars.utp.edu.my/id/eprint/34139/; https://www.scopus.com/inward/record.uri?eid=2-s2.0-85144440944&doi=10.1016%2fj.molliq.2022.120965&partnerID=40&md5=8bc8afa97a88789e9ebce1b57ffcd3db

Medium components and their solubility for improvement of recombinant phytase production in Escherichia coli DH5α. was optimized and reported in this study. Solubility study was supported by the computational works conducted using COSMO-RS, a quick tool to predict the thermophysical and chemical pr...