Comparative Analysis of Multiple Designs for Aqueous Two-Phase Protein Extraction Systems
A comparative analysis are made to compare flowsheet designs of the application of Aqueous Two-Phase System (ATPS) to extract protein from cell lysate. The analysis that observes the protein purity and yield trends is conducted using a flowsheet calculation framework that calculates the thermodynami...
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| Format: | Conference or Workshop Item |
| Published: |
2006
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| Online Access: | http://eprints.utp.edu.my/3232/1/Comparative_Analysis_of_Multiple_Designs_for_Aqueous_Two_Phase_Protein_Extraction_Systems_%28ARM_2006%29.pdf http://eprints.utp.edu.my/3232/ |
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| Summary: | A comparative analysis are made to compare flowsheet designs of the application of Aqueous Two-Phase System (ATPS) to extract protein from cell lysate. The analysis that observes the protein purity and yield trends is conducted using a flowsheet calculation framework that calculates the thermodynamic behaviour of the polymers and polymer-salt systems containing protein(s) within a limited range of properties and concentration. The designs include one-stage and two-stage extraction stages containing two types of ATPS i.e. PEG6000-DxT500 and PEG6000-Na3PO4 containing phosphofructokinase and ovalbumin. The results match previous findings to some extent. The analysis highlights potential for flowsheet design optimization work. |
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