Production of ligninolytic enzymes from thermophilic bacterial strains isolated from palm oil wastes
The conversion of lignocellulosic biomass into bioethanol or biochemical products requires a crucial pre-treatment process to break down the recalcitrant lignin structure. Biological depolymerization of lignin using microbial enzyme appeared to be a promising pre-treatment alternative as it offers e...
Saved in:
Main Authors: | , , , |
---|---|
Format: | Conference or Workshop Item |
Language: | English |
Published: |
2022
|
Subjects: | |
Online Access: | http://eprints.utm.my/id/eprint/98993/1/FazrenaNadiaMd2022_ProductionofLigninolyticEnzymesfromThermophilic.pdf http://eprints.utm.my/id/eprint/98993/ http://dx.doi.org/10.1088/1755-1315/1091/1/012063 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
my.utm.98993 |
---|---|
record_format |
eprints |
spelling |
my.utm.989932023-02-22T03:29:12Z http://eprints.utm.my/id/eprint/98993/ Production of ligninolytic enzymes from thermophilic bacterial strains isolated from palm oil wastes Riyadi, F. A. Azman, N. F. Yusof, N. Md. Akhir, Fazrena Nadia Q Science (General) TP Chemical technology The conversion of lignocellulosic biomass into bioethanol or biochemical products requires a crucial pre-treatment process to break down the recalcitrant lignin structure. Biological depolymerization of lignin using microbial enzyme appeared to be a promising pre-treatment alternative as it offers environmentally friendly treatment with lower energy requirements. In this study, three (3) thermophilic bacterial strains (S2, S11Y, S23) with lignin-degrading potential were previously isolated from palm oil wastes and identified as Stenotrophomonas sp., Bacillus subtilis, and Aeribacillus sp., respectively. These isolates demonstrated the capability to grow in a medium containing AL as the sole carbon source. Most isolates also demonstrated enzymatic activities toward lignin peroxidase (LiP), manganese peroxidase (MnP), and laccase (Lac) enzymes within a short period of 72 h. The highest LiP was secreted by strain S2 (70.97 ± 0.059 U/L), followed by S11Y (63.570 ± 0.021 U/L) and S23 (60.880 ± 0.042 U/L). MnP was generated with low activities of approximately 1.313 ± 0.037 U/L by S11Y, 0.364 ± 0.004 U/L by S2, and 0.215 ± 0.021 U/L by S23. For the Lac enzyme, low activities were detected only in S2 and S11Y, with 2.075 ± 0.612 U/L and 1.463 ± 0.116 U/L, respectively. This preliminary study appeared to show that the isolated thermophilic bacteria have the potential to be applied for lignin degradation application. 2022-11 Conference or Workshop Item PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/98993/1/FazrenaNadiaMd2022_ProductionofLigninolyticEnzymesfromThermophilic.pdf Riyadi, F. A. and Azman, N. F. and Yusof, N. and Md. Akhir, Fazrena Nadia (2022) Production of ligninolytic enzymes from thermophilic bacterial strains isolated from palm oil wastes. In: 9th AUN/SEED-Net Regional Conference on Natural Disaster, RCND 2021, 15 December 2021 - 16 December 2021, Virtual, Online. http://dx.doi.org/10.1088/1755-1315/1091/1/012063 |
institution |
Universiti Teknologi Malaysia |
building |
UTM Library |
collection |
Institutional Repository |
continent |
Asia |
country |
Malaysia |
content_provider |
Universiti Teknologi Malaysia |
content_source |
UTM Institutional Repository |
url_provider |
http://eprints.utm.my/ |
language |
English |
topic |
Q Science (General) TP Chemical technology |
spellingShingle |
Q Science (General) TP Chemical technology Riyadi, F. A. Azman, N. F. Yusof, N. Md. Akhir, Fazrena Nadia Production of ligninolytic enzymes from thermophilic bacterial strains isolated from palm oil wastes |
description |
The conversion of lignocellulosic biomass into bioethanol or biochemical products requires a crucial pre-treatment process to break down the recalcitrant lignin structure. Biological depolymerization of lignin using microbial enzyme appeared to be a promising pre-treatment alternative as it offers environmentally friendly treatment with lower energy requirements. In this study, three (3) thermophilic bacterial strains (S2, S11Y, S23) with lignin-degrading potential were previously isolated from palm oil wastes and identified as Stenotrophomonas sp., Bacillus subtilis, and Aeribacillus sp., respectively. These isolates demonstrated the capability to grow in a medium containing AL as the sole carbon source. Most isolates also demonstrated enzymatic activities toward lignin peroxidase (LiP), manganese peroxidase (MnP), and laccase (Lac) enzymes within a short period of 72 h. The highest LiP was secreted by strain S2 (70.97 ± 0.059 U/L), followed by S11Y (63.570 ± 0.021 U/L) and S23 (60.880 ± 0.042 U/L). MnP was generated with low activities of approximately 1.313 ± 0.037 U/L by S11Y, 0.364 ± 0.004 U/L by S2, and 0.215 ± 0.021 U/L by S23. For the Lac enzyme, low activities were detected only in S2 and S11Y, with 2.075 ± 0.612 U/L and 1.463 ± 0.116 U/L, respectively. This preliminary study appeared to show that the isolated thermophilic bacteria have the potential to be applied for lignin degradation application. |
format |
Conference or Workshop Item |
author |
Riyadi, F. A. Azman, N. F. Yusof, N. Md. Akhir, Fazrena Nadia |
author_facet |
Riyadi, F. A. Azman, N. F. Yusof, N. Md. Akhir, Fazrena Nadia |
author_sort |
Riyadi, F. A. |
title |
Production of ligninolytic enzymes from thermophilic bacterial strains isolated from palm oil wastes |
title_short |
Production of ligninolytic enzymes from thermophilic bacterial strains isolated from palm oil wastes |
title_full |
Production of ligninolytic enzymes from thermophilic bacterial strains isolated from palm oil wastes |
title_fullStr |
Production of ligninolytic enzymes from thermophilic bacterial strains isolated from palm oil wastes |
title_full_unstemmed |
Production of ligninolytic enzymes from thermophilic bacterial strains isolated from palm oil wastes |
title_sort |
production of ligninolytic enzymes from thermophilic bacterial strains isolated from palm oil wastes |
publishDate |
2022 |
url |
http://eprints.utm.my/id/eprint/98993/1/FazrenaNadiaMd2022_ProductionofLigninolyticEnzymesfromThermophilic.pdf http://eprints.utm.my/id/eprint/98993/ http://dx.doi.org/10.1088/1755-1315/1091/1/012063 |
_version_ |
1758578048199819264 |
score |
13.160551 |