Improved quantitative microbiome profiling for environmental antibiotic resistance surveillance

• Main Authors: Ott, Amelie, Quintela-Baluja, Marcos, Zealand, Andrew M., O’Donnell, Greg, Mohd. Haniffah, Mohd. Ridza, Graham, David W.
• Format: Article
• Language: English
• Published: BioMed Central Ltd 2021
• Subjects: TA Engineering (General). Civil engineering (General)
• Online Access: http://eprints.utm.my/id/eprint/95500/1/MohdRidzaMohd2021_ImprovedQuantitativeMicrobiomeProfiling.pdf; http://eprints.utm.my/id/eprint/95500/; http://dx.doi.org/10.1186/s40793-021-00391-0

Background: Understanding environmental microbiomes and antibiotic resistance (AR) is hindered by over reliance on relative abundance data from next-generation sequencing. Relative data limits our ability to quantify changes in microbiomes and resistomes over space and time because sequencing depth...