Structural and functional characterisation of a cold-active yet heat-tolerant dehydroquinase from glaciozyma antarctica pi12

• Main Authors: Jaafar, N. R., Mahadi, N. M., Mackeen, M. M., Illias, R. M., Murad, A. M. A., Bakar, F. D. A.
• Format: Article
• Published: Elsevier B.V. 2021
• Subjects: TP Chemical technology
• Online Access: http://eprints.utm.my/id/eprint/95248/; http://dx.doi.org/10.1016/j.jbiotec.2021.01.019

Dehydroquinase or 3-dehydroquinate dehydratase (DHQD) reversibly cleaves 3-dehydroquinate to form 3-dehydroshikimate. Here, we describe the functional and structural features of a cold active type II 3-dehydroquinate dehydratase from the psychrophilic yeast, Glaciozyma antarctica PI12 (GaDHQD). Func...