Bacterial disinfection and cell assessment post ultraviolet-C LED exposure for wound treatment

• Main Authors: Sheikh, Jahanzeb, Tian Swee, Tan, Saidin, Syafiqah, Yahya, Azli, Ahmed Malik, Sameen, Sia, Joyce Sin Yin, Tiong, Matthias Foh Thye
• Format: Article
• Published: Springer Science and Business Media Deutschland Gmb 2021
• Subjects: QD Chemistry
• Online Access: http://eprints.utm.my/id/eprint/95172/; http://dx.doi.org/10.1007/s11517-021-02360-8

Ultraviolet-C sourced LED (UVC-LED) has been widely used for disinfection purposes due to its germicidal spectrum. In this study, the efficiencies of UVC-LED for Pseudomonas aeruginosa (P. aeruginosa) and Staphylococcus aureus (S. aureus) disinfections were investigated at three exposure distances (1, 1.5, and 2 cm) and two exposure times (30 and 60 s). The respective bacterial inhibition zones were measured, followed by a morphological analysis under SEM. The viabilities of human skin fibroblast cells were further evaluated under the treatment of UVC-LED with the adoption of aforesaid exposure parameters. The inhibition zones were increased with the increment of exposure distances and times. The highest records of 5.40 ± 0.10 cm P. aeruginosa inhibition and 5.43 ± 0.11 cm S. aureus inhibition were observed at the UVC-LED distance of 2 cm and 60-s exposure. Bacterial physical damage with debris formation and reduction in size were visualized following the UVC-LED exposures. The cell viability percentages were in a range of 75.20–99.00% and 82–100.00% for the 30- and 60-s exposures, respectively. Thus, UVC-LED with 275-nm wavelength is capable in providing bacterial disinfection while maintaining accountable cell viability which is suitable to be adopted in wound treatment.H