Synthesis and characterization of silver-coated polymeric scaffolds for bone tissue engineering: antibacterial and in vitro evaluation of cytotoxicity and biocompatibility
In bone tissue engineering, multifunctional composite materials are very challenging. Bone tissue engineering is an innovative technique to develop biocompatible scaffolds with suitable orthopedic applications with enhanced antibacterial and mechanical properties. This research introduces a polymeri...
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my.utm.949392022-04-29T22:22:50Z http://eprints.utm.my/id/eprint/94939/ Synthesis and characterization of silver-coated polymeric scaffolds for bone tissue engineering: antibacterial and in vitro evaluation of cytotoxicity and biocompatibility Aslam Khan, Muhammad Umar Abd. Razak, Saiful Izwan Mehboob, Hassan Abdul Kadir, Mohammed Rafiq Anand, T. Joseph Sahaya Inam, Fawad Shah, Saqlain A. Abdel-Haliem, Mahmoud E. F. Amin, Rashid QH301 Biology In bone tissue engineering, multifunctional composite materials are very challenging. Bone tissue engineering is an innovative technique to develop biocompatible scaffolds with suitable orthopedic applications with enhanced antibacterial and mechanical properties. This research introduces a polymeric nanocomposite scaffold based on arabinoxylan-co-acrylic acid, nano-hydroxyapatite (nHAp), nano-aluminum oxide (nAl2O3), and graphene oxide (GO) by free-radical polymerization for the development of porous scaffolds using the freeze-drying technique. These polymeric nanocomposite scaffolds were coated with silver (Ag) nanoparticles to improve antibacterial activities. Together, nHAp, nAl2O3, and GO enhance the multifunctional properties of materials, which regulate their physicochemical and biomechanical properties. Results revealed that the Ag-coated polymeric nanocomposite scaffolds had excellent antibacterial properties and better microstructural properties. Regulated morphological properties and maximal antibacterial inhibition zones were found in the porous scaffolds with the increasing amount of GO. Moreover, the nanosystem and the polymeric matrix have improved the compressive strength (18.89 MPa) and Young's modulus (198.61 MPa) of scaffolds upon increasing the amount of GO. The biological activities of the scaffolds were investigated against the mouse preosteoblast cell lines (MC3T3-E1) and increasing the quantities of GO helps cell adherence and proliferation. Therefore, our findings showed that these silver-coated polymeric nanocomposite scaffolds have the potential for engineering bone tissue. American Chemical Society 2021 Article PeerReviewed Aslam Khan, Muhammad Umar and Abd. Razak, Saiful Izwan and Mehboob, Hassan and Abdul Kadir, Mohammed Rafiq and Anand, T. Joseph Sahaya and Inam, Fawad and Shah, Saqlain A. and Abdel-Haliem, Mahmoud E. F. and Amin, Rashid (2021) Synthesis and characterization of silver-coated polymeric scaffolds for bone tissue engineering: antibacterial and in vitro evaluation of cytotoxicity and biocompatibility. ACS Omega, 6 (6). pp. 4335-4346. ISSN 2470-1343 http://dx.doi.org/10.1021/acsomega.0c05596 |
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QH301 Biology Aslam Khan, Muhammad Umar Abd. Razak, Saiful Izwan Mehboob, Hassan Abdul Kadir, Mohammed Rafiq Anand, T. Joseph Sahaya Inam, Fawad Shah, Saqlain A. Abdel-Haliem, Mahmoud E. F. Amin, Rashid Synthesis and characterization of silver-coated polymeric scaffolds for bone tissue engineering: antibacterial and in vitro evaluation of cytotoxicity and biocompatibility |
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In bone tissue engineering, multifunctional composite materials are very challenging. Bone tissue engineering is an innovative technique to develop biocompatible scaffolds with suitable orthopedic applications with enhanced antibacterial and mechanical properties. This research introduces a polymeric nanocomposite scaffold based on arabinoxylan-co-acrylic acid, nano-hydroxyapatite (nHAp), nano-aluminum oxide (nAl2O3), and graphene oxide (GO) by free-radical polymerization for the development of porous scaffolds using the freeze-drying technique. These polymeric nanocomposite scaffolds were coated with silver (Ag) nanoparticles to improve antibacterial activities. Together, nHAp, nAl2O3, and GO enhance the multifunctional properties of materials, which regulate their physicochemical and biomechanical properties. Results revealed that the Ag-coated polymeric nanocomposite scaffolds had excellent antibacterial properties and better microstructural properties. Regulated morphological properties and maximal antibacterial inhibition zones were found in the porous scaffolds with the increasing amount of GO. Moreover, the nanosystem and the polymeric matrix have improved the compressive strength (18.89 MPa) and Young's modulus (198.61 MPa) of scaffolds upon increasing the amount of GO. The biological activities of the scaffolds were investigated against the mouse preosteoblast cell lines (MC3T3-E1) and increasing the quantities of GO helps cell adherence and proliferation. Therefore, our findings showed that these silver-coated polymeric nanocomposite scaffolds have the potential for engineering bone tissue. |
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Article |
author |
Aslam Khan, Muhammad Umar Abd. Razak, Saiful Izwan Mehboob, Hassan Abdul Kadir, Mohammed Rafiq Anand, T. Joseph Sahaya Inam, Fawad Shah, Saqlain A. Abdel-Haliem, Mahmoud E. F. Amin, Rashid |
author_facet |
Aslam Khan, Muhammad Umar Abd. Razak, Saiful Izwan Mehboob, Hassan Abdul Kadir, Mohammed Rafiq Anand, T. Joseph Sahaya Inam, Fawad Shah, Saqlain A. Abdel-Haliem, Mahmoud E. F. Amin, Rashid |
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Aslam Khan, Muhammad Umar |
title |
Synthesis and characterization of silver-coated polymeric scaffolds for bone tissue engineering: antibacterial and in vitro evaluation of cytotoxicity and biocompatibility |
title_short |
Synthesis and characterization of silver-coated polymeric scaffolds for bone tissue engineering: antibacterial and in vitro evaluation of cytotoxicity and biocompatibility |
title_full |
Synthesis and characterization of silver-coated polymeric scaffolds for bone tissue engineering: antibacterial and in vitro evaluation of cytotoxicity and biocompatibility |
title_fullStr |
Synthesis and characterization of silver-coated polymeric scaffolds for bone tissue engineering: antibacterial and in vitro evaluation of cytotoxicity and biocompatibility |
title_full_unstemmed |
Synthesis and characterization of silver-coated polymeric scaffolds for bone tissue engineering: antibacterial and in vitro evaluation of cytotoxicity and biocompatibility |
title_sort |
synthesis and characterization of silver-coated polymeric scaffolds for bone tissue engineering: antibacterial and in vitro evaluation of cytotoxicity and biocompatibility |
publisher |
American Chemical Society |
publishDate |
2021 |
url |
http://eprints.utm.my/id/eprint/94939/ http://dx.doi.org/10.1021/acsomega.0c05596 |
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1732945412528013312 |
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13.159267 |