Development of porous, antibacterial and biocompatible GO/n-HAp/bacterial cellulose/β-glucan biocomposite scaffold for bone tissue engineering
Due to their potential renewable materials-based tissue engineering scaffolds has gained more attention. Therefore, researchers are looking for new materials to be used as a scaffold. In this study, we have focused on the development of a nanocomposite scaffold for bone tissue engineering (using bac...
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my.utm.949192022-04-29T22:05:40Z http://eprints.utm.my/id/eprint/94919/ Development of porous, antibacterial and biocompatible GO/n-HAp/bacterial cellulose/β-glucan biocomposite scaffold for bone tissue engineering Khan, Muhammad Umar Aslam Haider, Sajjad Haider, Adnan Abd. Razak, Saiful Izwan Abdul Kadir, Mohammed Rafiq Shah, Saqlain A. Javed, Aneela Shakir, Imran Al-Zahrani, Ateyah A. QM Human anatomy Due to their potential renewable materials-based tissue engineering scaffolds has gained more attention. Therefore, researchers are looking for new materials to be used as a scaffold. In this study, we have focused on the development of a nanocomposite scaffold for bone tissue engineering (using bacterial cellulose (BC) and ß-glucan (ß-G)) via free radical polymerization and freeze-drying technique. Hydroxyapatite nanoparticles (n-HAp) and graphene oxide (GO) were added as reinforcement materials. The structural changes, surface morphology, porosity, and mechanical properties were investigated through spectroscopic and analytical techniques like Fourier transformation infrared (FT-IR), scanning electron microscope (SEM), Brunauer–Emmett-Teller (BET), and universal testing machine Instron. The scaffolds showed remarkable stability, aqueous degradation, spongy morphology, porosity, and mechanical properties. Antibacterial activities were performed against gram -ive and gram + ive bacterial strains. The BgC-1.4 scaffold was found more antibacterial compared to BgC-1.3, BgC-1.2, and BgC-1.1. The cell culture and cytotoxicity were evaluated using the MC3T3-E1 cell line. More cell growth was observed onto BgC-1.4 due to its uniform interrelated pores distribution, surface roughness, better mechanical properties, considerable biochemical affinity towards cell adhesion, proliferation, and biocompatibility. These nanocomposite scaffolds can be potential biomaterials for fractured bones in orthopedic tissue engineering. Elsevier B.V. 2021 Article PeerReviewed Khan, Muhammad Umar Aslam and Haider, Sajjad and Haider, Adnan and Abd. Razak, Saiful Izwan and Abdul Kadir, Mohammed Rafiq and Shah, Saqlain A. and Javed, Aneela and Shakir, Imran and Al-Zahrani, Ateyah A. (2021) Development of porous, antibacterial and biocompatible GO/n-HAp/bacterial cellulose/β-glucan biocomposite scaffold for bone tissue engineering. Arabian Journal of Chemistry, 14 (2). p. 102924. ISSN 1878-5352 http://dx.doi.org/10.1016/j.arabjc.2020.102924 |
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QM Human anatomy Khan, Muhammad Umar Aslam Haider, Sajjad Haider, Adnan Abd. Razak, Saiful Izwan Abdul Kadir, Mohammed Rafiq Shah, Saqlain A. Javed, Aneela Shakir, Imran Al-Zahrani, Ateyah A. Development of porous, antibacterial and biocompatible GO/n-HAp/bacterial cellulose/β-glucan biocomposite scaffold for bone tissue engineering |
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Due to their potential renewable materials-based tissue engineering scaffolds has gained more attention. Therefore, researchers are looking for new materials to be used as a scaffold. In this study, we have focused on the development of a nanocomposite scaffold for bone tissue engineering (using bacterial cellulose (BC) and ß-glucan (ß-G)) via free radical polymerization and freeze-drying technique. Hydroxyapatite nanoparticles (n-HAp) and graphene oxide (GO) were added as reinforcement materials. The structural changes, surface morphology, porosity, and mechanical properties were investigated through spectroscopic and analytical techniques like Fourier transformation infrared (FT-IR), scanning electron microscope (SEM), Brunauer–Emmett-Teller (BET), and universal testing machine Instron. The scaffolds showed remarkable stability, aqueous degradation, spongy morphology, porosity, and mechanical properties. Antibacterial activities were performed against gram -ive and gram + ive bacterial strains. The BgC-1.4 scaffold was found more antibacterial compared to BgC-1.3, BgC-1.2, and BgC-1.1. The cell culture and cytotoxicity were evaluated using the MC3T3-E1 cell line. More cell growth was observed onto BgC-1.4 due to its uniform interrelated pores distribution, surface roughness, better mechanical properties, considerable biochemical affinity towards cell adhesion, proliferation, and biocompatibility. These nanocomposite scaffolds can be potential biomaterials for fractured bones in orthopedic tissue engineering. |
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Article |
author |
Khan, Muhammad Umar Aslam Haider, Sajjad Haider, Adnan Abd. Razak, Saiful Izwan Abdul Kadir, Mohammed Rafiq Shah, Saqlain A. Javed, Aneela Shakir, Imran Al-Zahrani, Ateyah A. |
author_facet |
Khan, Muhammad Umar Aslam Haider, Sajjad Haider, Adnan Abd. Razak, Saiful Izwan Abdul Kadir, Mohammed Rafiq Shah, Saqlain A. Javed, Aneela Shakir, Imran Al-Zahrani, Ateyah A. |
author_sort |
Khan, Muhammad Umar Aslam |
title |
Development of porous, antibacterial and biocompatible GO/n-HAp/bacterial cellulose/β-glucan biocomposite scaffold for bone tissue engineering |
title_short |
Development of porous, antibacterial and biocompatible GO/n-HAp/bacterial cellulose/β-glucan biocomposite scaffold for bone tissue engineering |
title_full |
Development of porous, antibacterial and biocompatible GO/n-HAp/bacterial cellulose/β-glucan biocomposite scaffold for bone tissue engineering |
title_fullStr |
Development of porous, antibacterial and biocompatible GO/n-HAp/bacterial cellulose/β-glucan biocomposite scaffold for bone tissue engineering |
title_full_unstemmed |
Development of porous, antibacterial and biocompatible GO/n-HAp/bacterial cellulose/β-glucan biocomposite scaffold for bone tissue engineering |
title_sort |
development of porous, antibacterial and biocompatible go/n-hap/bacterial cellulose/β-glucan biocomposite scaffold for bone tissue engineering |
publisher |
Elsevier B.V. |
publishDate |
2021 |
url |
http://eprints.utm.my/id/eprint/94919/ http://dx.doi.org/10.1016/j.arabjc.2020.102924 |
_version_ |
1732945410108948480 |
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13.18916 |