Developing surrogate markers for predicting antibiotic resistance "hot spots" in rivers where limited data are available
Pinpointing environmental antibiotic resistance (AR) hot spots in low-and middle-income countries (LMICs) is hindered by a lack of available and comparable AR monitoring data relevant to such settings. Addressing this problem, we performed a comprehensive spatial and seasonal assessment of water qua...
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my.utm.942142022-03-31T15:24:51Z http://eprints.utm.my/id/eprint/94214/ Developing surrogate markers for predicting antibiotic resistance "hot spots" in rivers where limited data are available Ott, Amelie O’Donnell, Greg Tran, Ngoc Han Mohd. Haniffah, Mohd. Ridza Su, Jian Qiang Zealand, Andrew M. Gin, Karina Yew Hoong Goodson, Michaela L. Zhu, Yong Guan Graham, David W. TA Engineering (General). Civil engineering (General) Pinpointing environmental antibiotic resistance (AR) hot spots in low-and middle-income countries (LMICs) is hindered by a lack of available and comparable AR monitoring data relevant to such settings. Addressing this problem, we performed a comprehensive spatial and seasonal assessment of water quality and AR conditions in a Malaysian river catchment to identify potential "simple"surrogates that mirror elevated AR. We screened for resistant coliforms, 22 antibiotics, 287 AR genes and integrons, and routine water quality parameters, covering absolute concentrations and mass loadings. To understand relationships, we introduced standardized "effect sizes"(Cohen's D) for AR monitoring to improve comparability of field studies. Overall, water quality generally declined and environmental AR levels increased as one moved down the catchment without major seasonal variations, except total antibiotic concentrations that were higher in the dry season (Cohen's D > 0.8, P < 0.05). Among simple surrogates, dissolved oxygen (DO) most strongly correlated (inversely) with total AR gene concentrations (Spearman's ρ 0.81, P < 0.05). We suspect this results from minimally treated sewage inputs, which also contain AR bacteria and genes, depleting DO in the most impacted reaches. Thus, although DO is not a measure of AR, lower DO levels reflect wastewater inputs, flagging possible AR hot spots. DO measurement is inexpensive, already monitored in many catchments, and exists in many numerical water quality models (e.g., oxygen sag curves). Therefore, we propose combining DO data and prospective modeling to guide local interventions, especially in LMIC rivers with limited data. ACS Publications 2021-06-01 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/94214/1/MohdRidzaMohd2021_DevelopingSurrogateMarkersforPredicting.pdf Ott, Amelie and O’Donnell, Greg and Tran, Ngoc Han and Mohd. Haniffah, Mohd. Ridza and Su, Jian Qiang and Zealand, Andrew M. and Gin, Karina Yew Hoong and Goodson, Michaela L. and Zhu, Yong Guan and Graham, David W. (2021) Developing surrogate markers for predicting antibiotic resistance "hot spots" in rivers where limited data are available. Environmental Science and Technology, 55 (11). pp. 7466-7478. ISSN 0013-936X http://dx.doi.org/10.1021/acs.est.1c00939 DOI:10.1021/acs.est.1c00939 |
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TA Engineering (General). Civil engineering (General) Ott, Amelie O’Donnell, Greg Tran, Ngoc Han Mohd. Haniffah, Mohd. Ridza Su, Jian Qiang Zealand, Andrew M. Gin, Karina Yew Hoong Goodson, Michaela L. Zhu, Yong Guan Graham, David W. Developing surrogate markers for predicting antibiotic resistance "hot spots" in rivers where limited data are available |
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Pinpointing environmental antibiotic resistance (AR) hot spots in low-and middle-income countries (LMICs) is hindered by a lack of available and comparable AR monitoring data relevant to such settings. Addressing this problem, we performed a comprehensive spatial and seasonal assessment of water quality and AR conditions in a Malaysian river catchment to identify potential "simple"surrogates that mirror elevated AR. We screened for resistant coliforms, 22 antibiotics, 287 AR genes and integrons, and routine water quality parameters, covering absolute concentrations and mass loadings. To understand relationships, we introduced standardized "effect sizes"(Cohen's D) for AR monitoring to improve comparability of field studies. Overall, water quality generally declined and environmental AR levels increased as one moved down the catchment without major seasonal variations, except total antibiotic concentrations that were higher in the dry season (Cohen's D > 0.8, P < 0.05). Among simple surrogates, dissolved oxygen (DO) most strongly correlated (inversely) with total AR gene concentrations (Spearman's ρ 0.81, P < 0.05). We suspect this results from minimally treated sewage inputs, which also contain AR bacteria and genes, depleting DO in the most impacted reaches. Thus, although DO is not a measure of AR, lower DO levels reflect wastewater inputs, flagging possible AR hot spots. DO measurement is inexpensive, already monitored in many catchments, and exists in many numerical water quality models (e.g., oxygen sag curves). Therefore, we propose combining DO data and prospective modeling to guide local interventions, especially in LMIC rivers with limited data. |
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author |
Ott, Amelie O’Donnell, Greg Tran, Ngoc Han Mohd. Haniffah, Mohd. Ridza Su, Jian Qiang Zealand, Andrew M. Gin, Karina Yew Hoong Goodson, Michaela L. Zhu, Yong Guan Graham, David W. |
author_facet |
Ott, Amelie O’Donnell, Greg Tran, Ngoc Han Mohd. Haniffah, Mohd. Ridza Su, Jian Qiang Zealand, Andrew M. Gin, Karina Yew Hoong Goodson, Michaela L. Zhu, Yong Guan Graham, David W. |
author_sort |
Ott, Amelie |
title |
Developing surrogate markers for predicting antibiotic resistance "hot spots" in rivers where limited data are available |
title_short |
Developing surrogate markers for predicting antibiotic resistance "hot spots" in rivers where limited data are available |
title_full |
Developing surrogate markers for predicting antibiotic resistance "hot spots" in rivers where limited data are available |
title_fullStr |
Developing surrogate markers for predicting antibiotic resistance "hot spots" in rivers where limited data are available |
title_full_unstemmed |
Developing surrogate markers for predicting antibiotic resistance "hot spots" in rivers where limited data are available |
title_sort |
developing surrogate markers for predicting antibiotic resistance "hot spots" in rivers where limited data are available |
publisher |
ACS Publications |
publishDate |
2021 |
url |
http://eprints.utm.my/id/eprint/94214/1/MohdRidzaMohd2021_DevelopingSurrogateMarkersforPredicting.pdf http://eprints.utm.my/id/eprint/94214/ http://dx.doi.org/10.1021/acs.est.1c00939 |
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1729703140387717120 |
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13.160551 |