D-xylonic acid from recombinant E. coli BL21 (DE3): Comparison between shake flask and benchtop bioreactor fermentation
Production D-xylonic acid from recombinant E. coli BL21 (DE3) at a small scale has been successfully done several times. In an attempt to scale up the production process to a bigger scale, fermentation in bioreactor was conducted. The comparison on growth of recombinant E. coli BL21 (DE3) and produc...
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| Main Authors: | , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2020
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| Subjects: | |
| Online Access: | http://eprints.utm.my/id/eprint/94142/1/RosliMdIllias2020_DXylonicAcidfromRecombinantEcoliBL21.pdf http://eprints.utm.my/id/eprint/94142/ http://dx.doi.org/10.1088/1742-6596/1529/5/052073 |
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| Summary: | Production D-xylonic acid from recombinant E. coli BL21 (DE3) at a small scale has been successfully done several times. In an attempt to scale up the production process to a bigger scale, fermentation in bioreactor was conducted. The comparison on growth of recombinant E. coli BL21 (DE3) and production of D-xylonic acid in both shake flask and bioreactor was investigated. Higher specific growth rate of recombinant E. coli BL21 (DE3) and concentration of D-xylonic acid however, was obtained by fermentation in shake flask which is 0.273 h-1 and 9.06 gL-1 D-xylonic acid respectively. The result suggests several mistakes done during fermentation in bioreactor that causes low concentration of D-xylonic acid in bioreactor fermentation. Therefore, this calls up for improvement and better scale-up strategies in future work. |
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