Functional characterisation and product specificity of Endo-β-1,3-glucanase from alkalophilic bacterium, Bacillus lehensis G1

• Main Authors: Jaafar, Nardiah Rizwana, Mohamad Khoiri, Norhazlin, Ismail, Noor Faizah, Nik Mahmood, Nik Azmi, Abdul Murad, Abdul Munir, Abu Bakar, Farah Diba, Mat Yajit, Noor Liana, Md. Illias, Rosli
• Format: Article
• Published: Elsevier Inc. 2020
• Subjects: TP Chemical technology
• Online Access: http://eprints.utm.my/id/eprint/93778/; http://dx.doi.org/10.1016/j.enzmictec.2020.109625

Endo-β-1,3-glucanase from alkalophilic bacterium, Bacillus lehensis G1 (Blg32) composed of 284 amino acids with a predicted molecular mass of 31.6 kDa is expressed in Escherichia coli and purified to homogeneity. Herein, Blg32 characteristics, substrates and product specificity as well as structural...