Population data of 21 autosomal STR loci in Malaysian populations for human identification
The use of 21 autosomal STR loci for human identification has been gaining popularity throughout the world. It has been indicated that the forensic statistical parameters for supporting the use of 21 STR loci varied among different populations. Hitherto, such data for the diverse Malaysian populatio...
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my.utm.912032021-06-21T08:41:01Z http://eprints.utm.my/id/eprint/91203/ Population data of 21 autosomal STR loci in Malaysian populations for human identification Rashid, Mohd. Nor Azlan Mahat, Naji Arafat Khan, Hussein Omar Abdul Wahab, Roswanira Maarof, Hasmerya Ismail, Dzulkiflee Alwi, Aedrianee Reeza Syed Hassan, Sharifah Nany Rahayu Karmilla QD Chemistry The use of 21 autosomal STR loci for human identification has been gaining popularity throughout the world. It has been indicated that the forensic statistical parameters for supporting the use of 21 STR loci varied among different populations. Hitherto, such data for the diverse Malaysian populations remain unreported, rendering doubts in the court of law about its real ability for human identification in Malaysian population. Using the GlobalFiler™ Express PCR Amplification Kit, complete DNA profiles of 21 STR loci from buccal swabs of convicted Malaysian criminal (n = 570; 190 each for Malays, Chinese, and Indians) (by the year 2016–2017) were analyzed for their allele frequencies, exact test of Hardy-Weinberg equilibrium, observed and expected heterozygosity, power of discrimination, power of exclusion, match probability, and polymorphism information content. Most of the loci were found to be in the Hardy-Weinberg equilibrium after the Bonferroni correction. Being the most informative locus, SE33 demonstrated the highest power of discrimination and power of exclusion, indicating its usefulness to discriminate individuals. In contrast, TPOX had the lowest power of discrimination and power of exclusion, as well as being the less informative genetic locus for all Malaysian population studied here. The probabilities that two individuals would share the same DNA profiles among the Malaysian Malays, Chinese, and Indians, as well as in general Malaysian population, were 1.3713 × 10−25, 2.8822 × 10−25, 7.5668 × 10−26, and 1.0385 × 10−26, respectively. The results obtained here were found comparable with similar studies reported in other populations. Hence, its robustness for forensic human identification among the Malaysian populations is, therefore, statistically supported. Springer-Verlag GmbH Germany 2020-09-01 Article PeerReviewed Rashid, Mohd. Nor Azlan and Mahat, Naji Arafat and Khan, Hussein Omar and Abdul Wahab, Roswanira and Maarof, Hasmerya and Ismail, Dzulkiflee and Alwi, Aedrianee Reeza and Syed Hassan, Sharifah Nany Rahayu Karmilla (2020) Population data of 21 autosomal STR loci in Malaysian populations for human identification. International Journal of Legal Medicine, 134 (5). pp. 1675-1678. ISSN 0937-9827 http://dx.doi.org/10.1007/s00414-020-02279-z DOI:10.1007/s00414-020-02279-z |
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QD Chemistry Rashid, Mohd. Nor Azlan Mahat, Naji Arafat Khan, Hussein Omar Abdul Wahab, Roswanira Maarof, Hasmerya Ismail, Dzulkiflee Alwi, Aedrianee Reeza Syed Hassan, Sharifah Nany Rahayu Karmilla Population data of 21 autosomal STR loci in Malaysian populations for human identification |
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The use of 21 autosomal STR loci for human identification has been gaining popularity throughout the world. It has been indicated that the forensic statistical parameters for supporting the use of 21 STR loci varied among different populations. Hitherto, such data for the diverse Malaysian populations remain unreported, rendering doubts in the court of law about its real ability for human identification in Malaysian population. Using the GlobalFiler™ Express PCR Amplification Kit, complete DNA profiles of 21 STR loci from buccal swabs of convicted Malaysian criminal (n = 570; 190 each for Malays, Chinese, and Indians) (by the year 2016–2017) were analyzed for their allele frequencies, exact test of Hardy-Weinberg equilibrium, observed and expected heterozygosity, power of discrimination, power of exclusion, match probability, and polymorphism information content. Most of the loci were found to be in the Hardy-Weinberg equilibrium after the Bonferroni correction. Being the most informative locus, SE33 demonstrated the highest power of discrimination and power of exclusion, indicating its usefulness to discriminate individuals. In contrast, TPOX had the lowest power of discrimination and power of exclusion, as well as being the less informative genetic locus for all Malaysian population studied here. The probabilities that two individuals would share the same DNA profiles among the Malaysian Malays, Chinese, and Indians, as well as in general Malaysian population, were 1.3713 × 10−25, 2.8822 × 10−25, 7.5668 × 10−26, and 1.0385 × 10−26, respectively. The results obtained here were found comparable with similar studies reported in other populations. Hence, its robustness for forensic human identification among the Malaysian populations is, therefore, statistically supported. |
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Article |
author |
Rashid, Mohd. Nor Azlan Mahat, Naji Arafat Khan, Hussein Omar Abdul Wahab, Roswanira Maarof, Hasmerya Ismail, Dzulkiflee Alwi, Aedrianee Reeza Syed Hassan, Sharifah Nany Rahayu Karmilla |
author_facet |
Rashid, Mohd. Nor Azlan Mahat, Naji Arafat Khan, Hussein Omar Abdul Wahab, Roswanira Maarof, Hasmerya Ismail, Dzulkiflee Alwi, Aedrianee Reeza Syed Hassan, Sharifah Nany Rahayu Karmilla |
author_sort |
Rashid, Mohd. Nor Azlan |
title |
Population data of 21 autosomal STR loci in Malaysian populations for human identification |
title_short |
Population data of 21 autosomal STR loci in Malaysian populations for human identification |
title_full |
Population data of 21 autosomal STR loci in Malaysian populations for human identification |
title_fullStr |
Population data of 21 autosomal STR loci in Malaysian populations for human identification |
title_full_unstemmed |
Population data of 21 autosomal STR loci in Malaysian populations for human identification |
title_sort |
population data of 21 autosomal str loci in malaysian populations for human identification |
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Springer-Verlag GmbH Germany |
publishDate |
2020 |
url |
http://eprints.utm.my/id/eprint/91203/ http://dx.doi.org/10.1007/s00414-020-02279-z |
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