Zingiber officinale Roscoe prevents DNA damage and improves muscle performance and bone integrity in old Sprague Dawley rats
Age-related loss of skeletal muscle mass and strength or sarcopenia is attributed to the high level of oxidative stress and inadequate nutritional intake. The imbalance in oxidative status with increased production of free radicals results in damage to the DNA which leads to cell dysfunction. This s...
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2020
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my.utm.904242021-04-30T14:41:33Z http://eprints.utm.my/id/eprint/90424/ Zingiber officinale Roscoe prevents DNA damage and improves muscle performance and bone integrity in old Sprague Dawley rats Makpol, Suzana Abdul Sani, Nur Fathiah Hakimi, Nur Haleeda Ab. Rani, Nazirah Zakaria, Siti Nor Asyikin Abd. Rasid, Ahmad Fais Gunasekaran, Geetha Mohd. Sahardi, Nur Fatin Nabilah Tan, Jen Kit Abd. Ghafar, Norzana Mad Nordin, Mariam Firdhaus TP Chemical technology Age-related loss of skeletal muscle mass and strength or sarcopenia is attributed to the high level of oxidative stress and inadequate nutritional intake. The imbalance in oxidative status with increased production of free radicals results in damage to the DNA which leads to cell dysfunction. This study aimed to determine the effect of Zingiber officinale Roscoe (ginger) on muscle performance and bone integrity in Sprague Dawley (SD) rats. SD rats aged three (young), nine (adult), and twenty-one (old) months old were treated with either distilled water or ginger extract at a concentration of 200 mg/kg body weight (BW) daily for 3 months via oral gavage. Muscle performance was assessed at 0, 1, 2, and 3 months of treatment by measuring muscle strength, muscle function, and bone integrity while DNA damage was determined by comet assay. Muscle cell histology was analyzed by hematoxylin and eosin (H&E) staining. Young and adult ginger-treated rats showed a significant improvement in muscle strength after 3 months of supplementation. Bone mineral density (BMD) and bone mineral content (BMC) were increased while fat free mass (FMM) was decreased after 3 months of ginger supplementation in young rats but not changed in adult and old ginger supplemented groups. Interestingly, supplementation of ginger for 3 months to the old rats decreased the level of damaged DNA. Histological findings showed reduction in the size of muscle fibre and fascicles with heterogenous morphology of the muscle fibres indicating sarcopenia was evident in old rats. Treatment with ginger extract improved the histological changes even though there was evidence of cellular infiltration (mild inflammation) and dilated blood vessels. In conclusion, Z. officinale Roscoe prevents DNA damage and improves muscle performance and bone integrity in SD rats indicating its potential in alleviating oxidative stress in ageing and thus delaying sarcopenia progression. Hindawi Limited 2020 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/90424/1/MariamFirdhausMadNordin2020_ZingiberOfficinaleRoscoePreventsDNADamage.pdf Makpol, Suzana and Abdul Sani, Nur Fathiah and Hakimi, Nur Haleeda and Ab. Rani, Nazirah and Zakaria, Siti Nor Asyikin and Abd. Rasid, Ahmad Fais and Gunasekaran, Geetha and Mohd. Sahardi, Nur Fatin Nabilah and Tan, Jen Kit and Abd. Ghafar, Norzana and Mad Nordin, Mariam Firdhaus (2020) Zingiber officinale Roscoe prevents DNA damage and improves muscle performance and bone integrity in old Sprague Dawley rats. Evidence-based Complementary and Alternative Medicine, 2020 . p. 3823780. ISSN 1741-427X http://dx.doi.org/10.1155/2020/3823780 |
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TP Chemical technology Makpol, Suzana Abdul Sani, Nur Fathiah Hakimi, Nur Haleeda Ab. Rani, Nazirah Zakaria, Siti Nor Asyikin Abd. Rasid, Ahmad Fais Gunasekaran, Geetha Mohd. Sahardi, Nur Fatin Nabilah Tan, Jen Kit Abd. Ghafar, Norzana Mad Nordin, Mariam Firdhaus Zingiber officinale Roscoe prevents DNA damage and improves muscle performance and bone integrity in old Sprague Dawley rats |
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Age-related loss of skeletal muscle mass and strength or sarcopenia is attributed to the high level of oxidative stress and inadequate nutritional intake. The imbalance in oxidative status with increased production of free radicals results in damage to the DNA which leads to cell dysfunction. This study aimed to determine the effect of Zingiber officinale Roscoe (ginger) on muscle performance and bone integrity in Sprague Dawley (SD) rats. SD rats aged three (young), nine (adult), and twenty-one (old) months old were treated with either distilled water or ginger extract at a concentration of 200 mg/kg body weight (BW) daily for 3 months via oral gavage. Muscle performance was assessed at 0, 1, 2, and 3 months of treatment by measuring muscle strength, muscle function, and bone integrity while DNA damage was determined by comet assay. Muscle cell histology was analyzed by hematoxylin and eosin (H&E) staining. Young and adult ginger-treated rats showed a significant improvement in muscle strength after 3 months of supplementation. Bone mineral density (BMD) and bone mineral content (BMC) were increased while fat free mass (FMM) was decreased after 3 months of ginger supplementation in young rats but not changed in adult and old ginger supplemented groups. Interestingly, supplementation of ginger for 3 months to the old rats decreased the level of damaged DNA. Histological findings showed reduction in the size of muscle fibre and fascicles with heterogenous morphology of the muscle fibres indicating sarcopenia was evident in old rats. Treatment with ginger extract improved the histological changes even though there was evidence of cellular infiltration (mild inflammation) and dilated blood vessels. In conclusion, Z. officinale Roscoe prevents DNA damage and improves muscle performance and bone integrity in SD rats indicating its potential in alleviating oxidative stress in ageing and thus delaying sarcopenia progression. |
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Makpol, Suzana Abdul Sani, Nur Fathiah Hakimi, Nur Haleeda Ab. Rani, Nazirah Zakaria, Siti Nor Asyikin Abd. Rasid, Ahmad Fais Gunasekaran, Geetha Mohd. Sahardi, Nur Fatin Nabilah Tan, Jen Kit Abd. Ghafar, Norzana Mad Nordin, Mariam Firdhaus |
author_facet |
Makpol, Suzana Abdul Sani, Nur Fathiah Hakimi, Nur Haleeda Ab. Rani, Nazirah Zakaria, Siti Nor Asyikin Abd. Rasid, Ahmad Fais Gunasekaran, Geetha Mohd. Sahardi, Nur Fatin Nabilah Tan, Jen Kit Abd. Ghafar, Norzana Mad Nordin, Mariam Firdhaus |
author_sort |
Makpol, Suzana |
title |
Zingiber officinale Roscoe prevents DNA damage and improves muscle performance and bone integrity in old Sprague Dawley rats |
title_short |
Zingiber officinale Roscoe prevents DNA damage and improves muscle performance and bone integrity in old Sprague Dawley rats |
title_full |
Zingiber officinale Roscoe prevents DNA damage and improves muscle performance and bone integrity in old Sprague Dawley rats |
title_fullStr |
Zingiber officinale Roscoe prevents DNA damage and improves muscle performance and bone integrity in old Sprague Dawley rats |
title_full_unstemmed |
Zingiber officinale Roscoe prevents DNA damage and improves muscle performance and bone integrity in old Sprague Dawley rats |
title_sort |
zingiber officinale roscoe prevents dna damage and improves muscle performance and bone integrity in old sprague dawley rats |
publisher |
Hindawi Limited |
publishDate |
2020 |
url |
http://eprints.utm.my/id/eprint/90424/1/MariamFirdhausMadNordin2020_ZingiberOfficinaleRoscoePreventsDNADamage.pdf http://eprints.utm.my/id/eprint/90424/ http://dx.doi.org/10.1155/2020/3823780 |
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1698696934081953792 |
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13.159267 |