Improvement of cross-linking and stability on cross-linked enzyme aggregate (CLEA)-xylanase by protein surface engineering

• Main Authors: Abdul Wahab, Mohd. Khairul Hakimi, El-Enshasy, Hesham Ali, Abu Bakar, Farah Diba, Abdul Murad, Abdul Munir, Md. Jahim, Jamaliah, Md. Illias, Rosli
• Format: Article
• Published: Elsevier Ltd 2019
• Subjects: TP Chemical technology
• Online Access: http://eprints.utm.my/id/eprint/89547/; http://dx.doi.org/10.1016/j.procbio.2019.07.017

Cross-linked enzyme aggregate (CLEA) is a well-known enzyme immobilization technique that is efficient and cost-effective. In this study, we proposed a combination of the surface modification of an enzyme and CLEA to increase the number of free amino groups. Site-directed mutagenesis was applied to...