Purification and identification of novel cytotoxic oligopeptides from soft coral sarcophyton glaucum
Globally, peptide-based anticancer therapies have drawn much attention. Marine organisms are a reservoir of anticancer peptides that await discovery. In this study, we aimed to identify cytotoxic oligopeptides from Sarcophyton glaucum. Peptides were purified from among the S. glaucum hydrolysates pr...
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my.utm.875102020-11-08T04:05:28Z http://eprints.utm.my/id/eprint/87510/ Purification and identification of novel cytotoxic oligopeptides from soft coral sarcophyton glaucum Quah, Yixian Mohd. Ismail, Nor Ismaliza Lean, Jillian Sim Ooi Affendi, Yang Amri Abd. Manan, Fazilah Teh, Lai Kuan Wong, Fai Chu Chai, Tsun Thai Q Science (General) Globally, peptide-based anticancer therapies have drawn much attention. Marine organisms are a reservoir of anticancer peptides that await discovery. In this study, we aimed to identify cytotoxic oligopeptides from Sarcophyton glaucum. Peptides were purified from among the S. glaucum hydrolysates produced by alcalase, chymotrypsin, papain, and trypsin, guided by a methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay on the human cervical cancer (HeLa) cell line for cytotoxicity evaluation. Purification techniques adopted were membrane ultrafiltration, gel filtration chromatography, solid phase extraction (SPE), and reversed-phase high-performance liquid chromatography (RP-HPLC). Purified peptides were identified by de novo peptide sequencing. From papain hydrolysate, three peptide sequences were identified: AGAPGG, AERQ, and RDTQ (428.45, 502.53, and 518.53 Da, respectively). Peptides synthesized from these sequences exhibited cytotoxicity on HeLa cells with median effect concentration (EC 50 ) values of 8.6, 4.9, and 5.6 mmol/L, respectively, up to 5.8-fold stronger than the anticancer drug 5-fluorouracil. When tested at their respective EC 50 , AGAPGG, AERQ, and RDTQ showed only 16%, 25%, and 11% cytotoxicity to non-cancerous Hek293 cells, respectively. In conclusion, AERQ, AGAPGG, and RDTQ are promising candidates for future development as peptide-based anticancer drugs. Zhejiang University Press 2019-01-01 Article PeerReviewed Quah, Yixian and Mohd. Ismail, Nor Ismaliza and Lean, Jillian Sim Ooi and Affendi, Yang Amri and Abd. Manan, Fazilah and Teh, Lai Kuan and Wong, Fai Chu and Chai, Tsun Thai (2019) Purification and identification of novel cytotoxic oligopeptides from soft coral sarcophyton glaucum. Journal of Zhejiang University: Science B, 20 (1). pp. 59-70. ISSN 1673-1581 http://dx.doi.org/10.1631/jzus.B1700586 DOI:10.1631/jzus.B1700586 |
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Q Science (General) Quah, Yixian Mohd. Ismail, Nor Ismaliza Lean, Jillian Sim Ooi Affendi, Yang Amri Abd. Manan, Fazilah Teh, Lai Kuan Wong, Fai Chu Chai, Tsun Thai Purification and identification of novel cytotoxic oligopeptides from soft coral sarcophyton glaucum |
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Globally, peptide-based anticancer therapies have drawn much attention. Marine organisms are a reservoir of anticancer peptides that await discovery. In this study, we aimed to identify cytotoxic oligopeptides from Sarcophyton glaucum. Peptides were purified from among the S. glaucum hydrolysates produced by alcalase, chymotrypsin, papain, and trypsin, guided by a methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay on the human cervical cancer (HeLa) cell line for cytotoxicity evaluation. Purification techniques adopted were membrane ultrafiltration, gel filtration chromatography, solid phase extraction (SPE), and reversed-phase high-performance liquid chromatography (RP-HPLC). Purified peptides were identified by de novo peptide sequencing. From papain hydrolysate, three peptide sequences were identified: AGAPGG, AERQ, and RDTQ (428.45, 502.53, and 518.53 Da, respectively). Peptides synthesized from these sequences exhibited cytotoxicity on HeLa cells with median effect concentration (EC 50 ) values of 8.6, 4.9, and 5.6 mmol/L, respectively, up to 5.8-fold stronger than the anticancer drug 5-fluorouracil. When tested at their respective EC 50 , AGAPGG, AERQ, and RDTQ showed only 16%, 25%, and 11% cytotoxicity to non-cancerous Hek293 cells, respectively. In conclusion, AERQ, AGAPGG, and RDTQ are promising candidates for future development as peptide-based anticancer drugs. |
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Article |
author |
Quah, Yixian Mohd. Ismail, Nor Ismaliza Lean, Jillian Sim Ooi Affendi, Yang Amri Abd. Manan, Fazilah Teh, Lai Kuan Wong, Fai Chu Chai, Tsun Thai |
author_facet |
Quah, Yixian Mohd. Ismail, Nor Ismaliza Lean, Jillian Sim Ooi Affendi, Yang Amri Abd. Manan, Fazilah Teh, Lai Kuan Wong, Fai Chu Chai, Tsun Thai |
author_sort |
Quah, Yixian |
title |
Purification and identification of novel cytotoxic oligopeptides from soft coral sarcophyton glaucum |
title_short |
Purification and identification of novel cytotoxic oligopeptides from soft coral sarcophyton glaucum |
title_full |
Purification and identification of novel cytotoxic oligopeptides from soft coral sarcophyton glaucum |
title_fullStr |
Purification and identification of novel cytotoxic oligopeptides from soft coral sarcophyton glaucum |
title_full_unstemmed |
Purification and identification of novel cytotoxic oligopeptides from soft coral sarcophyton glaucum |
title_sort |
purification and identification of novel cytotoxic oligopeptides from soft coral sarcophyton glaucum |
publisher |
Zhejiang University Press |
publishDate |
2019 |
url |
http://eprints.utm.my/id/eprint/87510/ http://dx.doi.org/10.1631/jzus.B1700586 |
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1683230776590598144 |
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13.2014675 |