A comparison of analytical methods for measuring concentrations of 25-hydroxy vitamin D in biological samples

• Main Authors: Rezayi, Majid, Ghayour-Mobarhan, Majid, Tavakoly Sany, Seyedeh Belin, Fani, Mona, Avan, Amir, Pasdar, Zahra, Ferns, Gordon A., Abouzari-Lotf, Ebrahim, Amiri, Iraj S.
• Format: Article
• Published: Royal Society of Chemistry 2018
• Subjects: TP Chemical technology
• Online Access: http://eprints.utm.my/id/eprint/86751/; http://dx.doi.org/10.1039/c8ay02146e

Serum 25-hydroxyvitamin D (25(OH)D) is the principal circulating metabolite of vitamin D. Several methods for measuring serum 25(OH)D have been reported. These methods include chromatographic methods (GC-MS, HPLC, and LC-MS), competitive protein-binding assay (CPBA), radioimmunoassay (RIA) and autom...