Characterization of detergent compatible protease from halophilic Virgibacillus sp CD6
A halophilic bacterium, Virgibacillus sp. strain CD6, was isolated from salted fish and its extracellular protease was characterized. Protease production was found to be highest when yeast extract was used as nitrogen source for growth. The protease exhibited stability at wide range of salt concentr...
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my.utm.866632020-09-30T09:01:43Z http://eprints.utm.my/id/eprint/86663/ Characterization of detergent compatible protease from halophilic Virgibacillus sp CD6 Lam, Ming Quan Nik Mut, Nik Nurhidayu Thevarajoo, Suganthi Chen, S. J. Selvaratnam, C. Hussin, H. Jamaluddin, Haryati Chong, C. Q Science (General) A halophilic bacterium, Virgibacillus sp. strain CD6, was isolated from salted fish and its extracellular protease was characterized. Protease production was found to be highest when yeast extract was used as nitrogen source for growth. The protease exhibited stability at wide range of salt concentration (0–12.5%, w/v), temperatures (20–60 °C), and pH (4–10) with maximum activity at 10.0% (w/v) NaCl, 60 °C, pH 7 and 10, indicating its polyextremophilicity. The protease activity was enhanced in the presence of Mg2+, Mn2+, Cd2+, and Al3+ (107–122% relative activity), and with retention of activity > 80% for all of other metal ions examined (K+, Ca2+, Cu2+, Co2+, Ni2+, Zn2+, and Fe3+). Both PMSF and EDTA inhibited protease activity, denoting serine protease and metalloprotease properties, respectively. High stability (> 70%) was demonstrated in the presence of organic solvents and detergent constituents, and the extracellular protease from strain CD6 was also found to be compatible in commercial detergents. Proteinaceous stain removal efficacy revealed that crude protease of strain CD6 could significantly enhance the performance of commercial detergent. The protease from Virgibacillus sp. strain CD6 could serve as a promising alternative for various applications, especially in detergent industry. Springer Verlag 2018-02 Article PeerReviewed Lam, Ming Quan and Nik Mut, Nik Nurhidayu and Thevarajoo, Suganthi and Chen, S. J. and Selvaratnam, C. and Hussin, H. and Jamaluddin, Haryati and Chong, C. (2018) Characterization of detergent compatible protease from halophilic Virgibacillus sp CD6. 3 Biotech, 8 (2). p. 104. ISSN 2190-572X http://dx.doi.org/10.1007/s13205-018-1133-2 |
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Q Science (General) Lam, Ming Quan Nik Mut, Nik Nurhidayu Thevarajoo, Suganthi Chen, S. J. Selvaratnam, C. Hussin, H. Jamaluddin, Haryati Chong, C. Characterization of detergent compatible protease from halophilic Virgibacillus sp CD6 |
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A halophilic bacterium, Virgibacillus sp. strain CD6, was isolated from salted fish and its extracellular protease was characterized. Protease production was found to be highest when yeast extract was used as nitrogen source for growth. The protease exhibited stability at wide range of salt concentration (0–12.5%, w/v), temperatures (20–60 °C), and pH (4–10) with maximum activity at 10.0% (w/v) NaCl, 60 °C, pH 7 and 10, indicating its polyextremophilicity. The protease activity was enhanced in the presence of Mg2+, Mn2+, Cd2+, and Al3+ (107–122% relative activity), and with retention of activity > 80% for all of other metal ions examined (K+, Ca2+, Cu2+, Co2+, Ni2+, Zn2+, and Fe3+). Both PMSF and EDTA inhibited protease activity, denoting serine protease and metalloprotease properties, respectively. High stability (> 70%) was demonstrated in the presence of organic solvents and detergent constituents, and the extracellular protease from strain CD6 was also found to be compatible in commercial detergents. Proteinaceous stain removal efficacy revealed that crude protease of strain CD6 could significantly enhance the performance of commercial detergent. The protease from Virgibacillus sp. strain CD6 could serve as a promising alternative for various applications, especially in detergent industry. |
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Article |
author |
Lam, Ming Quan Nik Mut, Nik Nurhidayu Thevarajoo, Suganthi Chen, S. J. Selvaratnam, C. Hussin, H. Jamaluddin, Haryati Chong, C. |
author_facet |
Lam, Ming Quan Nik Mut, Nik Nurhidayu Thevarajoo, Suganthi Chen, S. J. Selvaratnam, C. Hussin, H. Jamaluddin, Haryati Chong, C. |
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Lam, Ming Quan |
title |
Characterization of detergent compatible protease from halophilic Virgibacillus sp CD6 |
title_short |
Characterization of detergent compatible protease from halophilic Virgibacillus sp CD6 |
title_full |
Characterization of detergent compatible protease from halophilic Virgibacillus sp CD6 |
title_fullStr |
Characterization of detergent compatible protease from halophilic Virgibacillus sp CD6 |
title_full_unstemmed |
Characterization of detergent compatible protease from halophilic Virgibacillus sp CD6 |
title_sort |
characterization of detergent compatible protease from halophilic virgibacillus sp cd6 |
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Springer Verlag |
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2018 |
url |
http://eprints.utm.my/id/eprint/86663/ http://dx.doi.org/10.1007/s13205-018-1133-2 |
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1680321078320168960 |
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13.160551 |