Elucidation of prazosin biodegradation by isolated bacillus spp. From the tropical environment

• Main Authors: Mohsi, N. F. M., Apandi, A., Noor, M. J. M. M., Akhir, F. N. M. D., Sugiura, N., Utsumi, M., Othman, N., Zakaria, Z., Hara, H.
• Format: Article
• Language: English
• Published: Microbiology Research Foundation 2020
• Subjects: T Technology (General)
• Online Access: http://eprints.utm.my/id/eprint/86071/1/HirofumiHara2020_ElucidationofPrazosinBiodegradationbyIsolatedBacillus.pdf; http://eprints.utm.my/id/eprint/86071/; https://dx.doi.org/10.2323/jgam.2019.04.001

Prazosin (PRZ), a drug used to treat hypertensive patients, is an emergent contaminant in water sys-tems. PRZ is an alpha-adrenergic receptor blocker used to treat anxiety, and is believed to reach the environment through human excretion, irrespon-sible disposal of unused medicine, and waste products from manufacturing plants. The purpose of this research was to isolate and characterize potential microbes for PRZ biodegradation and to identify the degradation pathway. After screening, isolated strain STP3 showed a capability for PRZ degradation and was chosen for further analysis. Resting cell assays with PRZ were conducted to identify the intermediate metabolites formed from biodegradation by Fourier transform ion cyclotron resonance mass spectrometer (FT-ICR MS) analy-sis. Two metabolites degraded from PRZ by STP3 were successfully found, and as these metabolites are derived from the main structure of PRZ, their presence proved PRZ degradation. Draft genome sequencing analysis of STP3 was performed to identify potential enzymes for PRZ biodegradation based on the metabolites found. © 2020 Applied Microbiology, Molecular and Cellular Biosciences Research Foundation.