Hemocyanin facilitates lignocellulose digestion by wood-boring marine crustaceans
Woody (lignocellulosic) plant biomass is an abundant renewable feedstock, rich in polysaccharides that are bound into an insoluble fiber composite with lignin. Marine crustacean woodborers of the genus Limnoria are among the few animals that can survive on a diet of this recalcitrant material withou...
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my.utm.859582020-08-30T08:46:25Z http://eprints.utm.my/id/eprint/85958/ Hemocyanin facilitates lignocellulose digestion by wood-boring marine crustaceans Besser, Katrin Malyon, Graham P. Eborall, William S. Paro da Cunha, Giovanni Filgueiras, Jefferson G. Dowle, Adam Garcia, Lourdes Cruz Page, Samuel J. Dupree, Ray Kern, Marcelo Gomez, Leonardo D. Li, Yi Elias, Luisa Sabbadin, Federico Mohamad, Shaza E. Pesante, Giovanna Steele-King, Clare Ribeiro de Azevedo, Eduardo Polikarpov, Igor Dupree, Paul Cragg, Simon M. Bruce, Neil C. McQueen-Mason, Simon J. T Technology (General) Woody (lignocellulosic) plant biomass is an abundant renewable feedstock, rich in polysaccharides that are bound into an insoluble fiber composite with lignin. Marine crustacean woodborers of the genus Limnoria are among the few animals that can survive on a diet of this recalcitrant material without relying on gut resident microbiota. Analysis of fecal pellets revealed that Limnoria targets hexose-containing polysaccharides (mainly cellulose, and also glucomannans), corresponding with the abundance of cellulases in their digestive system, but xylans and lignin are largely unconsumed. We show that the limnoriid respiratory protein, hemocyanin, is abundant in the hindgut where wood is digested, that incubation of wood with hemocyanin markedly enhances its digestibility by cellulases, and that it modifies lignin. We propose that this activity of hemocyanins is instrumental to the ability of Limnoria to feed on wood in the absence of gut symbionts. These findings may hold potential for innovations in lignocellulose biorefining. Nature Publishing Group 2018-12-03 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/85958/1/ShazaEMohamad2018_HemocyaninFacilitatesLignocelluloseDigestionByWood.pdf Besser, Katrin and Malyon, Graham P. and Eborall, William S. and Paro da Cunha, Giovanni and Filgueiras, Jefferson G. and Dowle, Adam and Garcia, Lourdes Cruz and Page, Samuel J. and Dupree, Ray and Kern, Marcelo and Gomez, Leonardo D. and Li, Yi and Elias, Luisa and Sabbadin, Federico and Mohamad, Shaza E. and Pesante, Giovanna and Steele-King, Clare and Ribeiro de Azevedo, Eduardo and Polikarpov, Igor and Dupree, Paul and Cragg, Simon M. and Bruce, Neil C. and McQueen-Mason, Simon J. (2018) Hemocyanin facilitates lignocellulose digestion by wood-boring marine crustaceans. Nature Communications, 9 (5125). pp. 1-14. ISSN 2041-1723 http://dx.doi.org/10.1038/s41467-018-07575-2 DOI:10.1038/s41467-018-07575-2 |
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T Technology (General) Besser, Katrin Malyon, Graham P. Eborall, William S. Paro da Cunha, Giovanni Filgueiras, Jefferson G. Dowle, Adam Garcia, Lourdes Cruz Page, Samuel J. Dupree, Ray Kern, Marcelo Gomez, Leonardo D. Li, Yi Elias, Luisa Sabbadin, Federico Mohamad, Shaza E. Pesante, Giovanna Steele-King, Clare Ribeiro de Azevedo, Eduardo Polikarpov, Igor Dupree, Paul Cragg, Simon M. Bruce, Neil C. McQueen-Mason, Simon J. Hemocyanin facilitates lignocellulose digestion by wood-boring marine crustaceans |
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Woody (lignocellulosic) plant biomass is an abundant renewable feedstock, rich in polysaccharides that are bound into an insoluble fiber composite with lignin. Marine crustacean woodborers of the genus Limnoria are among the few animals that can survive on a diet of this recalcitrant material without relying on gut resident microbiota. Analysis of fecal pellets revealed that Limnoria targets hexose-containing polysaccharides (mainly cellulose, and also glucomannans), corresponding with the abundance of cellulases in their digestive system, but xylans and lignin are largely unconsumed. We show that the limnoriid respiratory protein, hemocyanin, is abundant in the hindgut where wood is digested, that incubation of wood with hemocyanin markedly enhances its digestibility by cellulases, and that it modifies lignin. We propose that this activity of hemocyanins is instrumental to the ability of Limnoria to feed on wood in the absence of gut symbionts. These findings may hold potential for innovations in lignocellulose biorefining. |
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Article |
author |
Besser, Katrin Malyon, Graham P. Eborall, William S. Paro da Cunha, Giovanni Filgueiras, Jefferson G. Dowle, Adam Garcia, Lourdes Cruz Page, Samuel J. Dupree, Ray Kern, Marcelo Gomez, Leonardo D. Li, Yi Elias, Luisa Sabbadin, Federico Mohamad, Shaza E. Pesante, Giovanna Steele-King, Clare Ribeiro de Azevedo, Eduardo Polikarpov, Igor Dupree, Paul Cragg, Simon M. Bruce, Neil C. McQueen-Mason, Simon J. |
author_facet |
Besser, Katrin Malyon, Graham P. Eborall, William S. Paro da Cunha, Giovanni Filgueiras, Jefferson G. Dowle, Adam Garcia, Lourdes Cruz Page, Samuel J. Dupree, Ray Kern, Marcelo Gomez, Leonardo D. Li, Yi Elias, Luisa Sabbadin, Federico Mohamad, Shaza E. Pesante, Giovanna Steele-King, Clare Ribeiro de Azevedo, Eduardo Polikarpov, Igor Dupree, Paul Cragg, Simon M. Bruce, Neil C. McQueen-Mason, Simon J. |
author_sort |
Besser, Katrin |
title |
Hemocyanin facilitates lignocellulose digestion by wood-boring marine crustaceans |
title_short |
Hemocyanin facilitates lignocellulose digestion by wood-boring marine crustaceans |
title_full |
Hemocyanin facilitates lignocellulose digestion by wood-boring marine crustaceans |
title_fullStr |
Hemocyanin facilitates lignocellulose digestion by wood-boring marine crustaceans |
title_full_unstemmed |
Hemocyanin facilitates lignocellulose digestion by wood-boring marine crustaceans |
title_sort |
hemocyanin facilitates lignocellulose digestion by wood-boring marine crustaceans |
publisher |
Nature Publishing Group |
publishDate |
2018 |
url |
http://eprints.utm.my/id/eprint/85958/1/ShazaEMohamad2018_HemocyaninFacilitatesLignocelluloseDigestionByWood.pdf http://eprints.utm.my/id/eprint/85958/ http://dx.doi.org/10.1038/s41467-018-07575-2 |
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1677781109768716288 |
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13.160551 |