High quality of bacterial dna extraction from Corbicula fluminea (Müller, 1774) tissue in Kelantan

• Main Authors: Dee, Koh Han, Appalasamy, Suganthi, Md. Nasir, Siti Nor Aini, Abdullah, Faizuan, Mohamad Nor, Maryana, Rak, Aweng Eh
• Format: Article
• Language: English
• Published: Penerbit UTM Press 2020
• Subjects: QD Chemistry
• Online Access: http://eprints.utm.my/id/eprint/85584/1/FaizuanAbdullah2020_HighQualityofBacterialDnaExtraction.pdf; http://eprints.utm.my/id/eprint/85584/; https://mjfas.utm.my/index.php/mjfas/article/view/1503

Corbicula fluminea is a freshwater clam that is served as a popular traditional food in Kelantan, Malaysia. However, there are limited studies that report on high quality bacterial metagenome deoxyribonucleic acid (DNA) from C. fluminea. Therefore, this study compares the effectiveness and efficiency of conventional cetyltrimethylammonium bromide (CTAB) protocol, a commercial kit, and modified CTAB protocol for bacterial DNA extraction from the soft tissue surface of raw C. fluminea. The instruments used to examine the quality of the extracted bacterial DNA were DeNovix DS-11 spectrophotometer, gel electrophoresis machine, and UV transilluminator. The results showed that the bacterial DNA extracted from modified CTAB protocol had the highest purity and integrity with the A260/A280 ratio of 1.92 ± 0.01 and A260/A230 ratio of 1.83 ± 0.06 as well as the DNA band with minimum smear. This concludes that modified CTAB protocol is the best approach for the bacterial extraction from the C. fluminea.