Strategy in manipulating transglycosylation activity of glycosyl hydrolase for oligosaccharide production
Background: The increasing market demand for oligosaccharides has intensified the need for efficient biocatalysts. Glycosyl hydrolases (GHs) are still gaining popularity as biocatalyst for oligosaccharides synthesis owing to its simple reaction and high selectivity. Purpose: Over the years, research...
Saved in:
Main Authors: | , , |
---|---|
Format: | Article |
Published: |
Taylor and Francis Inc.
2018
|
Subjects: | |
Online Access: | http://eprints.utm.my/id/eprint/85363/ http://dx.doi.org/10.1080/07388551.2017.1339664 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
my.utm.85363 |
---|---|
record_format |
eprints |
spelling |
my.utm.853632020-03-17T08:27:06Z http://eprints.utm.my/id/eprint/85363/ Strategy in manipulating transglycosylation activity of glycosyl hydrolase for oligosaccharide production Abdul Manas, Nor Hasmaliana Md. Illias, Rosli Mahadi, Nor Muhammad TP Chemical technology Background: The increasing market demand for oligosaccharides has intensified the need for efficient biocatalysts. Glycosyl hydrolases (GHs) are still gaining popularity as biocatalyst for oligosaccharides synthesis owing to its simple reaction and high selectivity. Purpose: Over the years, research has advanced mainly directing to one goal; to reduce hydrolysis activity of GHs for increased transglycosylation activity in achieving high production of oligosaccharides. Design and methods: This review concisely presents the strategies to increase transglycosylation activity of GHs for oligosaccharides synthesis, focusing on controlling the reaction equilibrium, and protein engineering. Various modifications of the subsites of GHs have been demonstrated to significantly modulate the hydrolysis and transglycosylation activity of the enzymes. The clear insight of the roles of each amino acid in these sites provides a platform for designing an enzyme that could synthesize a specific oligosaccharide product. Conclusions: The key strategies presented here are important for future improvement of GHs as a biocatalyst for oligosaccharide synthesis. Taylor and Francis Inc. 2018-02 Article PeerReviewed Abdul Manas, Nor Hasmaliana and Md. Illias, Rosli and Mahadi, Nor Muhammad (2018) Strategy in manipulating transglycosylation activity of glycosyl hydrolase for oligosaccharide production. Critical Reviews in Biotechnology, 38 (2). pp. 272-293. ISSN 0738-8551 http://dx.doi.org/10.1080/07388551.2017.1339664 |
institution |
Universiti Teknologi Malaysia |
building |
UTM Library |
collection |
Institutional Repository |
continent |
Asia |
country |
Malaysia |
content_provider |
Universiti Teknologi Malaysia |
content_source |
UTM Institutional Repository |
url_provider |
http://eprints.utm.my/ |
topic |
TP Chemical technology |
spellingShingle |
TP Chemical technology Abdul Manas, Nor Hasmaliana Md. Illias, Rosli Mahadi, Nor Muhammad Strategy in manipulating transglycosylation activity of glycosyl hydrolase for oligosaccharide production |
description |
Background: The increasing market demand for oligosaccharides has intensified the need for efficient biocatalysts. Glycosyl hydrolases (GHs) are still gaining popularity as biocatalyst for oligosaccharides synthesis owing to its simple reaction and high selectivity. Purpose: Over the years, research has advanced mainly directing to one goal; to reduce hydrolysis activity of GHs for increased transglycosylation activity in achieving high production of oligosaccharides. Design and methods: This review concisely presents the strategies to increase transglycosylation activity of GHs for oligosaccharides synthesis, focusing on controlling the reaction equilibrium, and protein engineering. Various modifications of the subsites of GHs have been demonstrated to significantly modulate the hydrolysis and transglycosylation activity of the enzymes. The clear insight of the roles of each amino acid in these sites provides a platform for designing an enzyme that could synthesize a specific oligosaccharide product. Conclusions: The key strategies presented here are important for future improvement of GHs as a biocatalyst for oligosaccharide synthesis. |
format |
Article |
author |
Abdul Manas, Nor Hasmaliana Md. Illias, Rosli Mahadi, Nor Muhammad |
author_facet |
Abdul Manas, Nor Hasmaliana Md. Illias, Rosli Mahadi, Nor Muhammad |
author_sort |
Abdul Manas, Nor Hasmaliana |
title |
Strategy in manipulating transglycosylation activity of glycosyl hydrolase for oligosaccharide production |
title_short |
Strategy in manipulating transglycosylation activity of glycosyl hydrolase for oligosaccharide production |
title_full |
Strategy in manipulating transglycosylation activity of glycosyl hydrolase for oligosaccharide production |
title_fullStr |
Strategy in manipulating transglycosylation activity of glycosyl hydrolase for oligosaccharide production |
title_full_unstemmed |
Strategy in manipulating transglycosylation activity of glycosyl hydrolase for oligosaccharide production |
title_sort |
strategy in manipulating transglycosylation activity of glycosyl hydrolase for oligosaccharide production |
publisher |
Taylor and Francis Inc. |
publishDate |
2018 |
url |
http://eprints.utm.my/id/eprint/85363/ http://dx.doi.org/10.1080/07388551.2017.1339664 |
_version_ |
1662754388927578112 |
score |
13.160551 |