Extraction of nanosilica from oil palm leaves and its application as support for lipase immobilization
The study reports the preparation of a composite consisting of magnetite coated with nanosilica extracted from oil palm leaves (OPL) ash as nanosupports for immobilization of Candida rugosa lipase (CRL) and its application for the synthesis of butyl butyrate. Results of immobilization parameters sho...
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my.utm.846742020-02-27T04:52:28Z http://eprints.utm.my/id/eprint/84674/ Extraction of nanosilica from oil palm leaves and its application as support for lipase immobilization Onoja, Emmanuel Chandren, Sheela Abdul Razak, Fazira Ilyana Abdul Wahab, Roswanira QD Chemistry The study reports the preparation of a composite consisting of magnetite coated with nanosilica extracted from oil palm leaves (OPL) ash as nanosupports for immobilization of Candida rugosa lipase (CRL) and its application for the synthesis of butyl butyrate. Results of immobilization parameters showed that ∼ 80% of CRL (84.5 mg) initially offered was immobilized onto the surface of the nanosupports to yield a maximum protein loading and specific activity of 67.5 ± 0.72 mg/g and 320.8 ± 0.42 U/g of support, respectively. Surface topography, morphology as well as information on surface composition obtained by Raman spectroscopy, atomic force microscopy, field emission scanning electron microscopy and transmission electron microscopy showed that CRL was successfully immobilized onto the nanosupports, affirming its biocompatibility. Under optimal conditions (3.5 mg/mL protein loading, at 45 ℃ 3 h and molar ratio 2:1 (1-butanol:n-butyric acid) the CRL/Gl-A-SiO2-MNPs gave a maximum yield of 94 ± 0.24% butyl butyrate as compared to 84 ± 0.32% in the lyophilized CRL. CRL/Gl-A-SiO2-MNPs showed an extended operational stability, retaining 50% of its initial activity after 17 consecutive esterification cycles. The results indicated that OPL derived nanosilica coated on magnetite can potentially be employed as carrier for lipase immobilization in replacement of the non-renewable conventionalsilica sources. Elsevier B.V. 2018-10 Article PeerReviewed Onoja, Emmanuel and Chandren, Sheela and Abdul Razak, Fazira Ilyana and Abdul Wahab, Roswanira (2018) Extraction of nanosilica from oil palm leaves and its application as support for lipase immobilization. Journal of Biotechnology, 283 . pp. 81-96. ISSN 0168-1656 http://dx.doi.org/10.1016/j.jbiotec.2018.07.036 |
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QD Chemistry Onoja, Emmanuel Chandren, Sheela Abdul Razak, Fazira Ilyana Abdul Wahab, Roswanira Extraction of nanosilica from oil palm leaves and its application as support for lipase immobilization |
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The study reports the preparation of a composite consisting of magnetite coated with nanosilica extracted from oil palm leaves (OPL) ash as nanosupports for immobilization of Candida rugosa lipase (CRL) and its application for the synthesis of butyl butyrate. Results of immobilization parameters showed that ∼ 80% of CRL (84.5 mg) initially offered was immobilized onto the surface of the nanosupports to yield a maximum protein loading and specific activity of 67.5 ± 0.72 mg/g and 320.8 ± 0.42 U/g of support, respectively. Surface topography, morphology as well as information on surface composition obtained by Raman spectroscopy, atomic force microscopy, field emission scanning electron microscopy and transmission electron microscopy showed that CRL was successfully immobilized onto the nanosupports, affirming its biocompatibility. Under optimal conditions (3.5 mg/mL protein loading, at 45 ℃ 3 h and molar ratio 2:1 (1-butanol:n-butyric acid) the CRL/Gl-A-SiO2-MNPs gave a maximum yield of 94 ± 0.24% butyl butyrate as compared to 84 ± 0.32% in the lyophilized CRL. CRL/Gl-A-SiO2-MNPs showed an extended operational stability, retaining 50% of its initial activity after 17 consecutive esterification cycles. The results indicated that OPL derived nanosilica coated on magnetite can potentially be employed as carrier for lipase immobilization in replacement of the non-renewable conventionalsilica sources. |
format |
Article |
author |
Onoja, Emmanuel Chandren, Sheela Abdul Razak, Fazira Ilyana Abdul Wahab, Roswanira |
author_facet |
Onoja, Emmanuel Chandren, Sheela Abdul Razak, Fazira Ilyana Abdul Wahab, Roswanira |
author_sort |
Onoja, Emmanuel |
title |
Extraction of nanosilica from oil palm leaves and its application as support for lipase immobilization |
title_short |
Extraction of nanosilica from oil palm leaves and its application as support for lipase immobilization |
title_full |
Extraction of nanosilica from oil palm leaves and its application as support for lipase immobilization |
title_fullStr |
Extraction of nanosilica from oil palm leaves and its application as support for lipase immobilization |
title_full_unstemmed |
Extraction of nanosilica from oil palm leaves and its application as support for lipase immobilization |
title_sort |
extraction of nanosilica from oil palm leaves and its application as support for lipase immobilization |
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Elsevier B.V. |
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2018 |
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http://eprints.utm.my/id/eprint/84674/ http://dx.doi.org/10.1016/j.jbiotec.2018.07.036 |
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