Site-saturation mutagenesis of mutant L-asparaginase II signal peptide hydrophobic region for improved excretion of cyclodextrin glucanotransferase
The excretion of cyclodextrin glucanotransferase (CGTase) into the culture medium offers significant advantages over cytoplasmic expression. However, the limitation of Escherichia coli is its inability to excrete high amount of CGTase outside the cells. In this study, modification of the hydrophobic...
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| Main Authors: | , |
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| Format: | Article |
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Springer-Verlag
2017
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| Subjects: | |
| Online Access: | http://eprints.utm.my/id/eprint/81138/ http://dx.doi.org/10.1007/s10295-017-1980-6 |
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