Production of xylonic acid by recombinant Escherichia coli
In this paper, the effect of cultural condition on xylonic acid secretion by recombinant Escherichia coli (E. coli) was investigated. Then, the optimized cultural condition was determined to elevate xylonic acid secretion. To achieve this, analysis on xylonic acid secretion by using hydroxamate assa...
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| Main Authors: | , , |
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| Format: | Article |
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Universiti Teknologi Malaysia
2017
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| Subjects: | |
| Online Access: | http://eprints.utm.my/id/eprint/80697/ http://sps.utm.my/thecolloquium/files/2017/12/TC-12-1-3.pdf |
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| Summary: | In this paper, the effect of cultural condition on xylonic acid secretion by recombinant Escherichia coli (E. coli) was investigated. Then, the optimized cultural condition was determined to elevate xylonic acid secretion. To achieve this, analysis on xylonic acid secretion by using hydroxamate assay and High Performance Liquid Chromatography (HPLC) was performed. From the experimental works, it was understood that post induction temperature, inducer concentration, substrate concentration, medium pH and type of medium have affected the secretion of xylonic acid. |
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