Fabric for biomedical application

• Main Author: Abd. Jamil, Syazwani
• Format: Thesis
• Language: English
• Published: 2014
• Subjects: TP Chemical technology
• Online Access: http://eprints.utm.my/id/eprint/78044/1/SyazwaniAbdJamilMFBME20141.pdf; http://eprints.utm.my/id/eprint/78044/; http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:90308

In this study, cotton fabric was used as a main material in creating two devices designed for cell proliferation and cell based assay application. The first device, low cost wax-impregnated cotton fabric platform was created to resemble a commercially available 96 well plates. The usage of cotton fabric platform was investigated through the proliferation of cell HSF 1184 on the designed platform. Surface property of cotton fabric platform was analyzed through FTIR (Fourier Transform Infrared) whereas biocompatibility of the platform was investigated through MTT (3-(4,5- Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. HSF 1184 proliferation on cotton fabric platform was observed through LVSEM (Low Vacuum Scanning Electron Microscope) and Confocal Microscope. Second device, known as cotton fabric based cell assay device are comprise of a microfluidic pattern surrounded by a hydrophobic background on the surface of cotton fabric. Capillary force exerting on the interstitial spaces between woven threads and spun fibers on the surface of cotton fabric are utilize as a natural pump to draw media bearing a suspended cell. Two types of suspended cells are used in this study; (HSF Fibroblast 1184; size: >5μm) and (Hybridoma; size: 2-5μm). Weave structures of cotton fabric are utilized as a natural filter to isolate cells based on size difference. Suspended cell were stained and wicking movement of cells drawn by the capillary wicking of the media in the hydrophilic channel was observed. To conclude, in this study, the usage of cotton fabric as a raw material for biomedical application was described in device designed for cell culture and cell based assay application.