Global transcriptomic response of Anoxybacillus sp. SK 3-4 to aluminum exposure
Anoxybacillus sp. SK 3-4 is a Gram-positive, rod-shaped bacterium and a member of family Bacillaceae. We had previously reported that the strain is an aluminum resistant thermophilic bacterium. This is the first report to provide a detailed analysis of the global transcriptional response of Anoxybac...
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my.utm.764322018-04-30T13:23:49Z http://eprints.utm.my/id/eprint/76432/ Global transcriptomic response of Anoxybacillus sp. SK 3-4 to aluminum exposure Lim, J. C. Thevarajoo, S. Selvaratnam, C. Goh, K. M. Shamsir, M. S. Ibrahim, Z. Chong, C. S. TP Chemical technology Anoxybacillus sp. SK 3-4 is a Gram-positive, rod-shaped bacterium and a member of family Bacillaceae. We had previously reported that the strain is an aluminum resistant thermophilic bacterium. This is the first report to provide a detailed analysis of the global transcriptional response of Anoxybacillus when the cells were exposed to 600 mg L−1 of aluminum. The transcriptome was sequenced using Illumina MiSeq sequencer. Total of 708 genes were differentially expressed (fold change >2.00) with 316 genes were up-regulated while 347 genes were down-regulated, in comparing to control with no aluminum added in the culture. Based on Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, the majority of genes encoding for cell metabolism such as glycolysis, sulfur metabolism, cysteine and methionine metabolism were up-regulated; while most of the gene associated with tricarboxylic acid cycle (TCA cycle) and valine, leucine and isoleucine metabolism were down-regulated. In addition, a significant number of the genes encoding ABC transporters, metal ions transporters, and some stress response proteins were also differentially expressed following aluminum exposure. The findings provide further insight and help us to understand on the resistance of Anoxybacillus sp. SK 3-4 toward aluminium. Wiley-VCH Verlag 2017 Article PeerReviewed Lim, J. C. and Thevarajoo, S. and Selvaratnam, C. and Goh, K. M. and Shamsir, M. S. and Ibrahim, Z. and Chong, C. S. (2017) Global transcriptomic response of Anoxybacillus sp. SK 3-4 to aluminum exposure. Journal of Basic Microbiology, 57 (2). pp. 151-161. ISSN 0233-111X https://www.scopus.com/inward/record.uri?eid=2-s2.0-85004088975&doi=10.1002%2fjobm.201600494&partnerID=40&md5=ceca1d7fb6eddc57c01562cc36b81e1e DOI:10.1002/jobm.201600494 |
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TP Chemical technology Lim, J. C. Thevarajoo, S. Selvaratnam, C. Goh, K. M. Shamsir, M. S. Ibrahim, Z. Chong, C. S. Global transcriptomic response of Anoxybacillus sp. SK 3-4 to aluminum exposure |
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Anoxybacillus sp. SK 3-4 is a Gram-positive, rod-shaped bacterium and a member of family Bacillaceae. We had previously reported that the strain is an aluminum resistant thermophilic bacterium. This is the first report to provide a detailed analysis of the global transcriptional response of Anoxybacillus when the cells were exposed to 600 mg L−1 of aluminum. The transcriptome was sequenced using Illumina MiSeq sequencer. Total of 708 genes were differentially expressed (fold change >2.00) with 316 genes were up-regulated while 347 genes were down-regulated, in comparing to control with no aluminum added in the culture. Based on Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, the majority of genes encoding for cell metabolism such as glycolysis, sulfur metabolism, cysteine and methionine metabolism were up-regulated; while most of the gene associated with tricarboxylic acid cycle (TCA cycle) and valine, leucine and isoleucine metabolism were down-regulated. In addition, a significant number of the genes encoding ABC transporters, metal ions transporters, and some stress response proteins were also differentially expressed following aluminum exposure. The findings provide further insight and help us to understand on the resistance of Anoxybacillus sp. SK 3-4 toward aluminium. |
format |
Article |
author |
Lim, J. C. Thevarajoo, S. Selvaratnam, C. Goh, K. M. Shamsir, M. S. Ibrahim, Z. Chong, C. S. |
author_facet |
Lim, J. C. Thevarajoo, S. Selvaratnam, C. Goh, K. M. Shamsir, M. S. Ibrahim, Z. Chong, C. S. |
author_sort |
Lim, J. C. |
title |
Global transcriptomic response of Anoxybacillus sp. SK 3-4 to aluminum exposure |
title_short |
Global transcriptomic response of Anoxybacillus sp. SK 3-4 to aluminum exposure |
title_full |
Global transcriptomic response of Anoxybacillus sp. SK 3-4 to aluminum exposure |
title_fullStr |
Global transcriptomic response of Anoxybacillus sp. SK 3-4 to aluminum exposure |
title_full_unstemmed |
Global transcriptomic response of Anoxybacillus sp. SK 3-4 to aluminum exposure |
title_sort |
global transcriptomic response of anoxybacillus sp. sk 3-4 to aluminum exposure |
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Wiley-VCH Verlag |
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2017 |
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http://eprints.utm.my/id/eprint/76432/ https://www.scopus.com/inward/record.uri?eid=2-s2.0-85004088975&doi=10.1002%2fjobm.201600494&partnerID=40&md5=ceca1d7fb6eddc57c01562cc36b81e1e |
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