Effects of single and co-immobilization on the product specificity of type I pullulanase from Anoxybacillus sp. SK3-4

• Main Authors: Kahar, U. M., Chan, K. G., Sani, M. H., Mohd. Noh, N. I., Goh, K. M.
• Format: Article
• Published: Elsevier B.V. 2017
• Subjects: QH Natural history
• Online Access: http://eprints.utm.my/id/eprint/76332/; https://www.scopus.com/inward/record.uri?eid=2-s2.0-85020727575&doi=10.1016%2fj.ijbiomac.2017.06.054&partnerID=40&md5=dcb5e8113a281fc17fb80ed00e770584

Type I pullulanase from Anoxybacillus sp. SK3-4 (PulASK) is an unusual debranching enzyme that specifically hydrolyzes starch α-1,6 linkages at long branches producing oligosaccharides (≥G8), but is nonreactive against short branches; thus, incapable of producing reducing sugars (G1–G7). We report o...