Recombinant protein expression of Moringa oleifera lectin in methylotrophic yeast as active coagulant for sustainable high turbid water treatment
The natural coagulant Moringa oleifera lectin (MoL) as cationic protein is a promising candidate in coagulation process of water treatment plant. Introducing the gene encoding MoL into a host, Pichia pastoris, to secrete soluble recombinant protein is assessed in this study. Initial screening using...
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Japan Society for Bioscience Biotechnology and Agrochemistry
2017
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my.utm.754462018-03-22T11:10:01Z http://eprints.utm.my/id/eprint/75446/ Recombinant protein expression of Moringa oleifera lectin in methylotrophic yeast as active coagulant for sustainable high turbid water treatment Abd. Wahid, M. A. Megat Mohd. Noor, M. J. Goto, M. Sugiura, N. Othman, N. Zakaria, Z. Ahmad Mohammed, T. Jusoh, A. Hara, H. T Technology (General) The natural coagulant Moringa oleifera lectin (MoL) as cationic protein is a promising candidate in coagulation process of water treatment plant. Introducing the gene encoding MoL into a host, Pichia pastoris, to secrete soluble recombinant protein is assessed in this study. Initial screening using PCR confirmed the insertion of MoL gene, and SDS-PAGE analysis detected the MoL protein at 8 kDa. Cultured optimization showed the highest MoL protein at 520 mg/L was observed at 28 °C for 144 h of culturing by induction in 1% methanol. Approximately, 0.40 mg/mL of recombinant MoL protein showed 95 ± 2% turbidity removal of 1% kaolin suspension. In 0.1% kaolin suspension, the concentration of MoL at 10 μg/mL exhibits the highest turbidity reduction at 68 ± 1%. Thus, recombinant MoL protein from P. pastoris is an effective coagulant for water treatment. Japan Society for Bioscience Biotechnology and Agrochemistry 2017 Article PeerReviewed Abd. Wahid, M. A. and Megat Mohd. Noor, M. J. and Goto, M. and Sugiura, N. and Othman, N. and Zakaria, Z. and Ahmad Mohammed, T. and Jusoh, A. and Hara, H. (2017) Recombinant protein expression of Moringa oleifera lectin in methylotrophic yeast as active coagulant for sustainable high turbid water treatment. Bioscience, Biotechnology and Biochemistry, 81 (8). pp. 1642-1649. ISSN 0916-8451 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85024863152&doi=10.1080%2f09168451.2017.1329617&partnerID=40&md5=bd7b8f643e421095dcff4b65e0ac4215 |
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The natural coagulant Moringa oleifera lectin (MoL) as cationic protein is a promising candidate in coagulation process of water treatment plant. Introducing the gene encoding MoL into a host, Pichia pastoris, to secrete soluble recombinant protein is assessed in this study. Initial screening using PCR confirmed the insertion of MoL gene, and SDS-PAGE analysis detected the MoL protein at 8 kDa. Cultured optimization showed the highest MoL protein at 520 mg/L was observed at 28 °C for 144 h of culturing by induction in 1% methanol. Approximately, 0.40 mg/mL of recombinant MoL protein showed 95 ± 2% turbidity removal of 1% kaolin suspension. In 0.1% kaolin suspension, the concentration of MoL at 10 μg/mL exhibits the highest turbidity reduction at 68 ± 1%. Thus, recombinant MoL protein from P. pastoris is an effective coagulant for water treatment. |
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Article |
author |
Abd. Wahid, M. A. Megat Mohd. Noor, M. J. Goto, M. Sugiura, N. Othman, N. Zakaria, Z. Ahmad Mohammed, T. Jusoh, A. Hara, H. |
author_facet |
Abd. Wahid, M. A. Megat Mohd. Noor, M. J. Goto, M. Sugiura, N. Othman, N. Zakaria, Z. Ahmad Mohammed, T. Jusoh, A. Hara, H. |
author_sort |
Abd. Wahid, M. A. |
title |
Recombinant protein expression of Moringa oleifera lectin in methylotrophic yeast as active coagulant for sustainable high turbid water treatment |
title_short |
Recombinant protein expression of Moringa oleifera lectin in methylotrophic yeast as active coagulant for sustainable high turbid water treatment |
title_full |
Recombinant protein expression of Moringa oleifera lectin in methylotrophic yeast as active coagulant for sustainable high turbid water treatment |
title_fullStr |
Recombinant protein expression of Moringa oleifera lectin in methylotrophic yeast as active coagulant for sustainable high turbid water treatment |
title_full_unstemmed |
Recombinant protein expression of Moringa oleifera lectin in methylotrophic yeast as active coagulant for sustainable high turbid water treatment |
title_sort |
recombinant protein expression of moringa oleifera lectin in methylotrophic yeast as active coagulant for sustainable high turbid water treatment |
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Japan Society for Bioscience Biotechnology and Agrochemistry |
publishDate |
2017 |
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http://eprints.utm.my/id/eprint/75446/ https://www.scopus.com/inward/record.uri?eid=2-s2.0-85024863152&doi=10.1080%2f09168451.2017.1329617&partnerID=40&md5=bd7b8f643e421095dcff4b65e0ac4215 |
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1643657066716332032 |
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13.18916 |