PEDOT:PSS-containing nanohydroxyapatite/chitosan conductive bionanocomposite scaffold: fabrication and evaluation

• Main Authors: Lari, Alireza, Sun, Tao, Sultana, Naznin
• Format: Article
• Language: English
• Published: Hindawi Limited 2016
• Subjects: QH Natural history
• Online Access: http://eprints.utm.my/id/eprint/74626/1/NazninSultana2016_PEDOTPSS-containingnanohydroxyapatite.pdf; http://eprints.utm.my/id/eprint/74626/; https://www.scopus.com/inward/record.uri?eid=2-s2.0-84987757809&doi=10.1155%2f2016%2f9421203&partnerID=40&md5=c16e144a2309bfc7625cd1118055b355

Conductive poly(3,4-ethylenedioxythiophene)-poly(4-styrene sulfonate) (PEDOT:PSS) was incorporated into nanohydroxyapatite/chitosan (nHA/CS) composite scaffolds through a freezing and lyophilization technique. The bionanocomposite conductive scaffold was then characterized using several techniques. A scanning electron microscope image showed that the nHA and PEDOT:PSS were dispersed homogeneously in the chitosan matrix, which was also confirmed by energy-dispersive X-ray (EDX) analysis. The conductive properties were measured using a digital multimeter. The weight loss and water-uptake properties of the bionanocomposite scaffolds were studied in vitro. An in vitro cell cytotoxicity test was carried out using mouse fibroblast (L929) cells cultured onto the scaffolds. Using a freezing and lyophilization technique, it was possible to fabricate three-dimensional, highly porous, and interconnected PEDOT:PSS/nHA/CS scaffolds with good handling properties. The porosity was 74% and the scaffold's conductivity was 9.72 ± 0.78 μS. The surface roughness was increased with the incorporation of nHA and PEDOT:PSS into the CS scaffold. The compressive mechanical properties increased significantly with the incorporation of nHA but did not change significantly with the incorporation of PEDOT:PSS. The PEDOT:PSS-containing nHA/CS scaffold exhibited significantly higher cell attachment. The PEDOT:PSS/nHA/CS scaffold could be a potential bionanocomposite conductive scaffold for tissue engineering.