Commercial cellulases and hemicellulase performance towards oil palm empty fruit bunch (OPEFB) hydrolysis
In this work, commercial cellulases and hemicellulases were evaluated for their hydrolytic activity towards pretreated oil palm empty fruit bunches (OPEFB). A total of three commercial cellulase preparations, Novozyme Celluclast®, Novozyme Cellic®Ctec, Dupont Accellerase®1500, and a commercial hemic...
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American Institute of Physics Inc.
2016
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my.utm.729642017-11-18T00:51:23Z http://eprints.utm.my/id/eprint/72964/ Commercial cellulases and hemicellulase performance towards oil palm empty fruit bunch (OPEFB) hydrolysis Abdul Fattah, S. S. Mohamed, R. Jahim, J. M. Illias, R. M. Abu Bakar, F. D. Murad, A. M. A. TP Chemical technology In this work, commercial cellulases and hemicellulases were evaluated for their hydrolytic activity towards pretreated oil palm empty fruit bunches (OPEFB). A total of three commercial cellulase preparations, Novozyme Celluclast®, Novozyme Cellic®Ctec, Dupont Accellerase®1500, and a commercial hemicellulase preparation, Novozyme Cellic®Htec, were evaluated. The assays were performed either using the cellulase alone or cellulase in combination with the hemicellulase, formulated at different enzyme activity ratios. Among the three cellulases, the Novozyme Cellic®Ctec yielded the highest reducing sugars, whereby 32.9% hydrolysis yield of OPEFB was achieved. The addition of the commercial hemicellulase to Celluclast® and Accellerase®1500 enhanced OPEFB hydrolysis. However, the addition of the hemicellulase to Cellic®Ctec, failed to enhance the production of the reducing sugars. Nevertheless, the amount of reducing sugars produced using Cellic®Ctec alone was the highest when compared to other enzyme combinations. It can be concluded that among the three commercial cellulases evaluated, the Novozyme Cellic®Ctec is the best enzyme for OPEFB hydrolysis. American Institute of Physics Inc. 2016 Conference or Workshop Item PeerReviewed Abdul Fattah, S. S. and Mohamed, R. and Jahim, J. M. and Illias, R. M. and Abu Bakar, F. D. and Murad, A. M. A. (2016) Commercial cellulases and hemicellulase performance towards oil palm empty fruit bunch (OPEFB) hydrolysis. In: 2016 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2016, 13 April 2016 through 14 April 2016, Faculty of Science and Technology, Universiti Kebangsaan MalaysiaSelangor; Malaysia. https://www.scopus.com/inward/record.uri?eid=2-s2.0-85014593723&doi=10.1063%2f1.4966712&partnerID=40&md5=d0b61939833a9aac898244d642433284 |
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TP Chemical technology Abdul Fattah, S. S. Mohamed, R. Jahim, J. M. Illias, R. M. Abu Bakar, F. D. Murad, A. M. A. Commercial cellulases and hemicellulase performance towards oil palm empty fruit bunch (OPEFB) hydrolysis |
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In this work, commercial cellulases and hemicellulases were evaluated for their hydrolytic activity towards pretreated oil palm empty fruit bunches (OPEFB). A total of three commercial cellulase preparations, Novozyme Celluclast®, Novozyme Cellic®Ctec, Dupont Accellerase®1500, and a commercial hemicellulase preparation, Novozyme Cellic®Htec, were evaluated. The assays were performed either using the cellulase alone or cellulase in combination with the hemicellulase, formulated at different enzyme activity ratios. Among the three cellulases, the Novozyme Cellic®Ctec yielded the highest reducing sugars, whereby 32.9% hydrolysis yield of OPEFB was achieved. The addition of the commercial hemicellulase to Celluclast® and Accellerase®1500 enhanced OPEFB hydrolysis. However, the addition of the hemicellulase to Cellic®Ctec, failed to enhance the production of the reducing sugars. Nevertheless, the amount of reducing sugars produced using Cellic®Ctec alone was the highest when compared to other enzyme combinations. It can be concluded that among the three commercial cellulases evaluated, the Novozyme Cellic®Ctec is the best enzyme for OPEFB hydrolysis. |
format |
Conference or Workshop Item |
author |
Abdul Fattah, S. S. Mohamed, R. Jahim, J. M. Illias, R. M. Abu Bakar, F. D. Murad, A. M. A. |
author_facet |
Abdul Fattah, S. S. Mohamed, R. Jahim, J. M. Illias, R. M. Abu Bakar, F. D. Murad, A. M. A. |
author_sort |
Abdul Fattah, S. S. |
title |
Commercial cellulases and hemicellulase performance towards oil palm empty fruit bunch (OPEFB) hydrolysis |
title_short |
Commercial cellulases and hemicellulase performance towards oil palm empty fruit bunch (OPEFB) hydrolysis |
title_full |
Commercial cellulases and hemicellulase performance towards oil palm empty fruit bunch (OPEFB) hydrolysis |
title_fullStr |
Commercial cellulases and hemicellulase performance towards oil palm empty fruit bunch (OPEFB) hydrolysis |
title_full_unstemmed |
Commercial cellulases and hemicellulase performance towards oil palm empty fruit bunch (OPEFB) hydrolysis |
title_sort |
commercial cellulases and hemicellulase performance towards oil palm empty fruit bunch (opefb) hydrolysis |
publisher |
American Institute of Physics Inc. |
publishDate |
2016 |
url |
http://eprints.utm.my/id/eprint/72964/ https://www.scopus.com/inward/record.uri?eid=2-s2.0-85014593723&doi=10.1063%2f1.4966712&partnerID=40&md5=d0b61939833a9aac898244d642433284 |
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1643656538070450176 |
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13.18916 |