Incrassamarin A–D: four new 4-substituted coumarins from Calophyllum incrassatum and their biological activities

• Main Authors: Aminudin, N. I., Ahmad, F., Taher, M., Zulkifli, R. M.
• Format: Article
• Published: Elsevier Ltd 2016
• Subjects: QD Chemistry
• Online Access: http://eprints.utm.my/id/eprint/72482/; https://www.scopus.com/inward/record.uri?eid=2-s2.0-84977620941&doi=10.1016%2fj.phytol.2016.05.008&partnerID=40&md5=44356757370cb55ac5c84d785302817f

Four new 4-substituted coumarins, incrassamarin A (1), B (2), C (3) and D (4) with (7S,8S)-7,8-dihydro-5-hydroxy-7,8-dimethyl-4-propyl-2H,6H-benzo[1,2-b;5,4-b’]dipyran-2,6-dione (5), friedelin, carpachromene, amentoflavone, epiafzelechin and L-quercitrin were isolated from the barks and leaves of Calophyllum incrassatum (Guttiferae). The compounds were isolated and purified by size-exclusion recycling HPLC and column chromatographic techniques. The structures of the compounds were determined by spectroscopic means. The compounds were tested for their cytotoxic activity towards MCF-7 and A-549 cell lines and α-glucosidase enzymatic inhibitory activity. Compound (1) displayed cytotoxic activity against A-549 cell lines with IC50 87.71 μg/mL and showed inhibition towards α-glucosidase enzymatic activity with IC50 93.25 μM. This is the first report on the isolation of phytochemicals from the barks and leaves of C. incrassatum and their bioactivities.