Structural and functional analysis of a novel psychrophilic beta-mannanase from Glaciozyma antarctica PI12
The structure of a novel psychrophilic beta-mannanase enzyme from Glaciozyma antarctica PI12 yeast has been modelled and analysed in detail. To our knowledge, this is the first attempt to model a psychrophilic beta-mannanase from yeast. To this end, a 3D structure of the enzyme was first predicted u...
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my.utm.626832017-06-01T06:11:57Z http://eprints.utm.my/id/eprint/62683/ Structural and functional analysis of a novel psychrophilic beta-mannanase from Glaciozyma antarctica PI12 Parvizpour, Sepideh Razmara, Jafar Ramli, Aizi Nor Mazila Md. Illias, Rosli Shamsir, Mohd. Shahir TP Chemical technology The structure of a novel psychrophilic beta-mannanase enzyme from Glaciozyma antarctica PI12 yeast has been modelled and analysed in detail. To our knowledge, this is the first attempt to model a psychrophilic beta-mannanase from yeast. To this end, a 3D structure of the enzyme was first predicted using a threading method because of the low sequence identity (< 30 %) using MODELLER9v12 and simulated using GROMACS at varying low temperatures for structure refinement. Comparisons with mesophilic and thermophilic mannanases revealed that the psychrophilic mannanase contains longer loops and shorter helices, increases in the number of aromatic and hydrophobic residues, reductions in the number of hydrogen bonds and salt bridges and numerous amino acid substitutions on the surface that increased the flexibility and its efficiency for catalytic reactions at low temperatures. Springer 2014 Article PeerReviewed Parvizpour, Sepideh and Razmara, Jafar and Ramli, Aizi Nor Mazila and Md. Illias, Rosli and Shamsir, Mohd. Shahir (2014) Structural and functional analysis of a novel psychrophilic beta-mannanase from Glaciozyma antarctica PI12. Journal of Computer-Aided Molecular Design, 28 (6). pp. 685-698. ISSN 0920-654X http://dx.doi.org/10.1007/s10822-014-9751-1 DOI:10.1007/s10822-014-9751-1 |
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TP Chemical technology Parvizpour, Sepideh Razmara, Jafar Ramli, Aizi Nor Mazila Md. Illias, Rosli Shamsir, Mohd. Shahir Structural and functional analysis of a novel psychrophilic beta-mannanase from Glaciozyma antarctica PI12 |
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The structure of a novel psychrophilic beta-mannanase enzyme from Glaciozyma antarctica PI12 yeast has been modelled and analysed in detail. To our knowledge, this is the first attempt to model a psychrophilic beta-mannanase from yeast. To this end, a 3D structure of the enzyme was first predicted using a threading method because of the low sequence identity (< 30 %) using MODELLER9v12 and simulated using GROMACS at varying low temperatures for structure refinement. Comparisons with mesophilic and thermophilic mannanases revealed that the psychrophilic mannanase contains longer loops and shorter helices, increases in the number of aromatic and hydrophobic residues, reductions in the number of hydrogen bonds and salt bridges and numerous amino acid substitutions on the surface that increased the flexibility and its efficiency for catalytic reactions at low temperatures. |
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Article |
author |
Parvizpour, Sepideh Razmara, Jafar Ramli, Aizi Nor Mazila Md. Illias, Rosli Shamsir, Mohd. Shahir |
author_facet |
Parvizpour, Sepideh Razmara, Jafar Ramli, Aizi Nor Mazila Md. Illias, Rosli Shamsir, Mohd. Shahir |
author_sort |
Parvizpour, Sepideh |
title |
Structural and functional analysis of a novel psychrophilic beta-mannanase from Glaciozyma antarctica PI12 |
title_short |
Structural and functional analysis of a novel psychrophilic beta-mannanase from Glaciozyma antarctica PI12 |
title_full |
Structural and functional analysis of a novel psychrophilic beta-mannanase from Glaciozyma antarctica PI12 |
title_fullStr |
Structural and functional analysis of a novel psychrophilic beta-mannanase from Glaciozyma antarctica PI12 |
title_full_unstemmed |
Structural and functional analysis of a novel psychrophilic beta-mannanase from Glaciozyma antarctica PI12 |
title_sort |
structural and functional analysis of a novel psychrophilic beta-mannanase from glaciozyma antarctica pi12 |
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Springer |
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2014 |
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http://eprints.utm.my/id/eprint/62683/ http://dx.doi.org/10.1007/s10822-014-9751-1 |
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13.2014675 |