Optimization of growth medium and procesing condition of acinetobacter sp as biological phosphorus removal for wastewater treatment in semi-industrial scale bioreactor

Acinetobacter sp. has been reported previously in their role to enhance the removal of biological phosphorus and heavy metal when introduced in waste water treatment. This is an importance characteristics to be used in remediating the waste water instead of depend on naturally present of microbes. T...

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Main Authors: Mohammad, Norhafizah, Hanapi, Siti Zulaiha, Abd. Malek, Roslinda, Velayutham, Sasidharan, Syed Mohamed, Syed Yassin, Lim, Pek Boon, Mohd. Aris, Alijah, Othman, NorZalina, Aziz, Ramlan, El Enshasy, Hesham
格式: Conference or Workshop Item
語言:English
出版: 2014
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在線閱讀:http://eprints.utm.my/id/eprint/61351/1/HeshamAliMetwally2014_OptimizationofGrowthMediumandProcesingCondition.pdf
http://eprints.utm.my/id/eprint/61351/
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spelling my.utm.613512017-03-31T01:31:57Z http://eprints.utm.my/id/eprint/61351/ Optimization of growth medium and procesing condition of acinetobacter sp as biological phosphorus removal for wastewater treatment in semi-industrial scale bioreactor Mohammad, Norhafizah Hanapi, Siti Zulaiha Abd. Malek, Roslinda Velayutham, Sasidharan Syed Mohamed, Syed Yassin Lim, Pek Boon Mohd. Aris, Alijah Othman, NorZalina Aziz, Ramlan El Enshasy, Hesham TP Chemical technology Acinetobacter sp. has been reported previously in their role to enhance the removal of biological phosphorus and heavy metal when introduced in waste water treatment. This is an importance characteristics to be used in remediating the waste water instead of depend on naturally present of microbes. Therefore, high densities of Acinetobacter sp. to function in the bioremediation treatment are needed. The optimization of Acinetobacter sp. was carried out in shake flask for 24 hours cultivation using different carbon sources (glucose, sucrose, maltose, fructose and glycerol), nitrogen sources (yeast extract, soy powder, corn steep liquor, beef extract and peptone from casein) and phosphate salts (potassium monohydrogen phosphate). Total cell mass of 2.14 g L-1 was produced in un-optimized semi-defined medium using glucose as sole carbon source. Replacement of glucose with sucrose resulted in the 62.60% increase of cell mass production (3.48 g L-1). Yeast extract (20 g L-1) was demonstrated as the best nitrogen source based on the 172.89 % increase of cell mass (5.84 g L-1). Unfortunately, different concentration of phosphate salts did not show any differences in cell mass production. A combination of the optimized parameters resulted in 7.59 g L-1 of cell mass after 16 hours of cultivation. In conclusion, Acinetobacter sp. showed cell mass production rate of 0.23 g L-1 h-1 under un-controlled pH compared to controlled pH (0.12 g L-1 h-1). 2014 Conference or Workshop Item PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/61351/1/HeshamAliMetwally2014_OptimizationofGrowthMediumandProcesingCondition.pdf Mohammad, Norhafizah and Hanapi, Siti Zulaiha and Abd. Malek, Roslinda and Velayutham, Sasidharan and Syed Mohamed, Syed Yassin and Lim, Pek Boon and Mohd. Aris, Alijah and Othman, NorZalina and Aziz, Ramlan and El Enshasy, Hesham (2014) Optimization of growth medium and procesing condition of acinetobacter sp as biological phosphorus removal for wastewater treatment in semi-industrial scale bioreactor. In: 5th International Conference on Biotechnology for the Wellness Industry (ICBWI), 10-11 Jun, 2014, Kuala Lumpur, Malaysia.
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Mohammad, Norhafizah
Hanapi, Siti Zulaiha
Abd. Malek, Roslinda
Velayutham, Sasidharan
Syed Mohamed, Syed Yassin
Lim, Pek Boon
Mohd. Aris, Alijah
Othman, NorZalina
Aziz, Ramlan
El Enshasy, Hesham
Optimization of growth medium and procesing condition of acinetobacter sp as biological phosphorus removal for wastewater treatment in semi-industrial scale bioreactor
description Acinetobacter sp. has been reported previously in their role to enhance the removal of biological phosphorus and heavy metal when introduced in waste water treatment. This is an importance characteristics to be used in remediating the waste water instead of depend on naturally present of microbes. Therefore, high densities of Acinetobacter sp. to function in the bioremediation treatment are needed. The optimization of Acinetobacter sp. was carried out in shake flask for 24 hours cultivation using different carbon sources (glucose, sucrose, maltose, fructose and glycerol), nitrogen sources (yeast extract, soy powder, corn steep liquor, beef extract and peptone from casein) and phosphate salts (potassium monohydrogen phosphate). Total cell mass of 2.14 g L-1 was produced in un-optimized semi-defined medium using glucose as sole carbon source. Replacement of glucose with sucrose resulted in the 62.60% increase of cell mass production (3.48 g L-1). Yeast extract (20 g L-1) was demonstrated as the best nitrogen source based on the 172.89 % increase of cell mass (5.84 g L-1). Unfortunately, different concentration of phosphate salts did not show any differences in cell mass production. A combination of the optimized parameters resulted in 7.59 g L-1 of cell mass after 16 hours of cultivation. In conclusion, Acinetobacter sp. showed cell mass production rate of 0.23 g L-1 h-1 under un-controlled pH compared to controlled pH (0.12 g L-1 h-1).
format Conference or Workshop Item
author Mohammad, Norhafizah
Hanapi, Siti Zulaiha
Abd. Malek, Roslinda
Velayutham, Sasidharan
Syed Mohamed, Syed Yassin
Lim, Pek Boon
Mohd. Aris, Alijah
Othman, NorZalina
Aziz, Ramlan
El Enshasy, Hesham
author_facet Mohammad, Norhafizah
Hanapi, Siti Zulaiha
Abd. Malek, Roslinda
Velayutham, Sasidharan
Syed Mohamed, Syed Yassin
Lim, Pek Boon
Mohd. Aris, Alijah
Othman, NorZalina
Aziz, Ramlan
El Enshasy, Hesham
author_sort Mohammad, Norhafizah
title Optimization of growth medium and procesing condition of acinetobacter sp as biological phosphorus removal for wastewater treatment in semi-industrial scale bioreactor
title_short Optimization of growth medium and procesing condition of acinetobacter sp as biological phosphorus removal for wastewater treatment in semi-industrial scale bioreactor
title_full Optimization of growth medium and procesing condition of acinetobacter sp as biological phosphorus removal for wastewater treatment in semi-industrial scale bioreactor
title_fullStr Optimization of growth medium and procesing condition of acinetobacter sp as biological phosphorus removal for wastewater treatment in semi-industrial scale bioreactor
title_full_unstemmed Optimization of growth medium and procesing condition of acinetobacter sp as biological phosphorus removal for wastewater treatment in semi-industrial scale bioreactor
title_sort optimization of growth medium and procesing condition of acinetobacter sp as biological phosphorus removal for wastewater treatment in semi-industrial scale bioreactor
publishDate 2014
url http://eprints.utm.my/id/eprint/61351/1/HeshamAliMetwally2014_OptimizationofGrowthMediumandProcesingCondition.pdf
http://eprints.utm.my/id/eprint/61351/
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