In vitro permeation and skin retention of alpha-mangostin proniosome
Alpha-mangostin has been identified as a potent anti-melanogenesis compound in vitro on B16F1 melanoma cells. A concentration of 5 µg/mL demonstrated promising anti-melanogenesis effect without compromising the cell viability. However, due to its high lipophilic nature, the cosmeceutical application...
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my.utm.607022020-12-22T07:47:25Z http://eprints.utm.my/id/eprint/60702/ In vitro permeation and skin retention of alpha-mangostin proniosome Gan, Siaw Chin TP Chemical technology Alpha-mangostin has been identified as a potent anti-melanogenesis compound in vitro on B16F1 melanoma cells. A concentration of 5 µg/mL demonstrated promising anti-melanogenesis effect without compromising the cell viability. However, due to its high lipophilic nature, the cosmeceutical application of a-mangostin in topical formulation is restricted. The current investigation aimed to evaluate the potential of proniosome as a carrier to enhance skin permeation and skin retention of a-mangostin. Alpha-mangostin proniosomal formulations were prepared using coacervation phase separation method. Upon hydration, a-mangostin-loaded niosomes were characterized for size, polydispersity index, entrapment efficiency, zeta potential and morphology. Using different surfactants, preliminary study to evaluate skin concentration suggested that Spans significantly (p < 0.05) enhanced deposition of a-mangostin in the viable epidermis/dermis layer (VED) as compared to Tween 60. Incorporation of soya lecithin in the proniosomal formulation also significantly enhanced the VED concentration of a-mangostin. The in vitro permeation experiments using dermis-split Yucatan Micropig skin revealed that proniosomes composed of Spans, soya lecithin and cholesterol were able to enhance the skin permeation of a-mangostin with a factor range from 1.8 to 8.0-fold as compared to the control suspension. All the proniosomal formulations (except for S20L) had significantly (p < 0.05) enhanced the deposition of a-mangostin in the VED layer with a factor range from 2.5 to 2.9-fold as compared to the control suspension. Proniosome S85L showed the highest permeation profile (8.0-fold) and the highest enhancement of VED concentration of a-mangostin (2.9-fold). Collectively, these results suggested that proniosomes can be utilized as a promising carrier for a highly lipophilic compound like a-mangostin. 2016-05 Thesis NonPeerReviewed application/pdf en http://eprints.utm.my/id/eprint/60702/1/GanSiawChinMFChE2016.pdf Gan, Siaw Chin (2016) In vitro permeation and skin retention of alpha-mangostin proniosome. Masters thesis, Universiti Teknologi Malaysia, Faculty of Chemical Engineering. http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:93953 |
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TP Chemical technology Gan, Siaw Chin In vitro permeation and skin retention of alpha-mangostin proniosome |
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Alpha-mangostin has been identified as a potent anti-melanogenesis compound in vitro on B16F1 melanoma cells. A concentration of 5 µg/mL demonstrated promising anti-melanogenesis effect without compromising the cell viability. However, due to its high lipophilic nature, the cosmeceutical application of a-mangostin in topical formulation is restricted. The current investigation aimed to evaluate the potential of proniosome as a carrier to enhance skin permeation and skin retention of a-mangostin. Alpha-mangostin proniosomal formulations were prepared using coacervation phase separation method. Upon hydration, a-mangostin-loaded niosomes were characterized for size, polydispersity index, entrapment efficiency, zeta potential and morphology. Using different surfactants, preliminary study to evaluate skin concentration suggested that Spans significantly (p < 0.05) enhanced deposition of a-mangostin in the viable epidermis/dermis layer (VED) as compared to Tween 60. Incorporation of soya lecithin in the proniosomal formulation also significantly enhanced the VED concentration of a-mangostin. The in vitro permeation experiments using dermis-split Yucatan Micropig skin revealed that proniosomes composed of Spans, soya lecithin and cholesterol were able to enhance the skin permeation of a-mangostin with a factor range from 1.8 to 8.0-fold as compared to the control suspension. All the proniosomal formulations (except for S20L) had significantly (p < 0.05) enhanced the deposition of a-mangostin in the VED layer with a factor range from 2.5 to 2.9-fold as compared to the control suspension. Proniosome S85L showed the highest permeation profile (8.0-fold) and the highest enhancement of VED concentration of a-mangostin (2.9-fold). Collectively, these results suggested that proniosomes can be utilized as a promising carrier for a highly lipophilic compound like a-mangostin. |
format |
Thesis |
author |
Gan, Siaw Chin |
author_facet |
Gan, Siaw Chin |
author_sort |
Gan, Siaw Chin |
title |
In vitro permeation and skin retention of alpha-mangostin proniosome |
title_short |
In vitro permeation and skin retention of alpha-mangostin proniosome |
title_full |
In vitro permeation and skin retention of alpha-mangostin proniosome |
title_fullStr |
In vitro permeation and skin retention of alpha-mangostin proniosome |
title_full_unstemmed |
In vitro permeation and skin retention of alpha-mangostin proniosome |
title_sort |
in vitro permeation and skin retention of alpha-mangostin proniosome |
publishDate |
2016 |
url |
http://eprints.utm.my/id/eprint/60702/1/GanSiawChinMFChE2016.pdf http://eprints.utm.my/id/eprint/60702/ http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:93953 |
_version_ |
1687393514285957120 |
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13.211869 |