In vitro permeation and skin retention of alpha-mangostin proniosome

Alpha-mangostin has been identified as a potent anti-melanogenesis compound in vitro on B16F1 melanoma cells. A concentration of 5 µg/mL demonstrated promising anti-melanogenesis effect without compromising the cell viability. However, due to its high lipophilic nature, the cosmeceutical application...

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Main Author: Gan, Siaw Chin
Format: Thesis
Language:English
Published: 2016
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Online Access:http://eprints.utm.my/id/eprint/60702/1/GanSiawChinMFChE2016.pdf
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spelling my.utm.607022020-12-22T07:47:25Z http://eprints.utm.my/id/eprint/60702/ In vitro permeation and skin retention of alpha-mangostin proniosome Gan, Siaw Chin TP Chemical technology Alpha-mangostin has been identified as a potent anti-melanogenesis compound in vitro on B16F1 melanoma cells. A concentration of 5 µg/mL demonstrated promising anti-melanogenesis effect without compromising the cell viability. However, due to its high lipophilic nature, the cosmeceutical application of a-mangostin in topical formulation is restricted. The current investigation aimed to evaluate the potential of proniosome as a carrier to enhance skin permeation and skin retention of a-mangostin. Alpha-mangostin proniosomal formulations were prepared using coacervation phase separation method. Upon hydration, a-mangostin-loaded niosomes were characterized for size, polydispersity index, entrapment efficiency, zeta potential and morphology. Using different surfactants, preliminary study to evaluate skin concentration suggested that Spans significantly (p < 0.05) enhanced deposition of a-mangostin in the viable epidermis/dermis layer (VED) as compared to Tween 60. Incorporation of soya lecithin in the proniosomal formulation also significantly enhanced the VED concentration of a-mangostin. The in vitro permeation experiments using dermis-split Yucatan Micropig skin revealed that proniosomes composed of Spans, soya lecithin and cholesterol were able to enhance the skin permeation of a-mangostin with a factor range from 1.8 to 8.0-fold as compared to the control suspension. All the proniosomal formulations (except for S20L) had significantly (p < 0.05) enhanced the deposition of a-mangostin in the VED layer with a factor range from 2.5 to 2.9-fold as compared to the control suspension. Proniosome S85L showed the highest permeation profile (8.0-fold) and the highest enhancement of VED concentration of a-mangostin (2.9-fold). Collectively, these results suggested that proniosomes can be utilized as a promising carrier for a highly lipophilic compound like a-mangostin. 2016-05 Thesis NonPeerReviewed application/pdf en http://eprints.utm.my/id/eprint/60702/1/GanSiawChinMFChE2016.pdf Gan, Siaw Chin (2016) In vitro permeation and skin retention of alpha-mangostin proniosome. Masters thesis, Universiti Teknologi Malaysia, Faculty of Chemical Engineering. http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:93953
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Gan, Siaw Chin
In vitro permeation and skin retention of alpha-mangostin proniosome
description Alpha-mangostin has been identified as a potent anti-melanogenesis compound in vitro on B16F1 melanoma cells. A concentration of 5 µg/mL demonstrated promising anti-melanogenesis effect without compromising the cell viability. However, due to its high lipophilic nature, the cosmeceutical application of a-mangostin in topical formulation is restricted. The current investigation aimed to evaluate the potential of proniosome as a carrier to enhance skin permeation and skin retention of a-mangostin. Alpha-mangostin proniosomal formulations were prepared using coacervation phase separation method. Upon hydration, a-mangostin-loaded niosomes were characterized for size, polydispersity index, entrapment efficiency, zeta potential and morphology. Using different surfactants, preliminary study to evaluate skin concentration suggested that Spans significantly (p < 0.05) enhanced deposition of a-mangostin in the viable epidermis/dermis layer (VED) as compared to Tween 60. Incorporation of soya lecithin in the proniosomal formulation also significantly enhanced the VED concentration of a-mangostin. The in vitro permeation experiments using dermis-split Yucatan Micropig skin revealed that proniosomes composed of Spans, soya lecithin and cholesterol were able to enhance the skin permeation of a-mangostin with a factor range from 1.8 to 8.0-fold as compared to the control suspension. All the proniosomal formulations (except for S20L) had significantly (p < 0.05) enhanced the deposition of a-mangostin in the VED layer with a factor range from 2.5 to 2.9-fold as compared to the control suspension. Proniosome S85L showed the highest permeation profile (8.0-fold) and the highest enhancement of VED concentration of a-mangostin (2.9-fold). Collectively, these results suggested that proniosomes can be utilized as a promising carrier for a highly lipophilic compound like a-mangostin.
format Thesis
author Gan, Siaw Chin
author_facet Gan, Siaw Chin
author_sort Gan, Siaw Chin
title In vitro permeation and skin retention of alpha-mangostin proniosome
title_short In vitro permeation and skin retention of alpha-mangostin proniosome
title_full In vitro permeation and skin retention of alpha-mangostin proniosome
title_fullStr In vitro permeation and skin retention of alpha-mangostin proniosome
title_full_unstemmed In vitro permeation and skin retention of alpha-mangostin proniosome
title_sort in vitro permeation and skin retention of alpha-mangostin proniosome
publishDate 2016
url http://eprints.utm.my/id/eprint/60702/1/GanSiawChinMFChE2016.pdf
http://eprints.utm.my/id/eprint/60702/
http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:93953
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score 13.211869