Overexpression, purification and characterization of aspergillus niger beta-glucosidase in pichia pastoris

• Main Authors: Kamaruddin, Shazilah, Abu Bakar, Farah Diba, Illias, Rosli Md., Said, Mamot, Hassan, Osman, Abdul Murad, Abdul Munir
• Format: Article
• Published: Malaysian Society of Applied Biology 2015
• Subjects: Q Science (General)
• Online Access: http://eprints.utm.my/id/eprint/58742/

This study describes the expression of ß-glucosidase (BglA) from Aspergillus niger in Pichia pastoris, a methylotrophic yeast strain, under the regulation of an alcohol oxidase promoter. The heterologous expression of BglA was optimized in a shake flask. Optimal conditions were achieved using an ini...