Model-aided anaerobic metabolic gene knockout of malate dehydrogenase (mdh) gene predicts increased succinate production in Escherichia coli
Succinic acid is considers to be a platform chemical with divergent applications as a precursor for syntheses of commodity and specialty chemicals. Its biobased production could be a green technology when produced by microbial fermentation using Escherichia coli as a chassis host. Metabolic eng...
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| Main Authors: | , |
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| Format: | Article |
| Published: |
2015
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| Subjects: | |
| Online Access: | http://eprints.utm.my/id/eprint/58572/ http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:99480 |
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| Summary: | Succinic acid is considers to be a platform chemical with
divergent applications as a precursor for syntheses of
commodity and specialty chemicals. Its biobased production
could be a green technology when produced by
microbial fermentation using Escherichia coli as a chassis
host. Metabolic engineering for increased production
of succinic acid using glucose as substrate is beset with
limitation of NADH availability under anaerobic
conditions in E. coli. In the present work, we used the
most recent genome scale metabolic model of E. coli
iJ01366 (Orth et al. 2011) to predict metabolic gene
knockout of mdh/b3236 with the aim of increasing
NADH availability using the glucose substrate. |
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