Diversity of thermophiles in a Malaysian hot spring determined using 16S rRNA and shotgun metagenome sequencing
The Sungai Klah (SK) hot spring is the second hottest geothermal spring in Malaysia. This hot spring is a shallow, 150-m-long, fast-flowing stream, with temperatures varying from 50 to 110°C and a pH range of 7.0-9.0. Hidden within a wooded area, the SK hot spring is continually fed by plant litter,...
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my.utm.583242021-12-14T04:56:44Z http://eprints.utm.my/id/eprint/58324/ Diversity of thermophiles in a Malaysian hot spring determined using 16S rRNA and shotgun metagenome sequencing Chan, Chia Sing Chan, Kok Gan Tay, Yea Ling Chua, Yi Heng Goh, Kian Mau Q Science (General) The Sungai Klah (SK) hot spring is the second hottest geothermal spring in Malaysia. This hot spring is a shallow, 150-m-long, fast-flowing stream, with temperatures varying from 50 to 110°C and a pH range of 7.0-9.0. Hidden within a wooded area, the SK hot spring is continually fed by plant litter, resulting in a relatively high degree of total organic content (TOC). In this study, a sample taken from the middle of the stream was analyzed at the 16S rRNA V3-V4 region by amplicon metagenome sequencing. Over 35 phyla were detected by analyzing the 16S rRNA data. Firmicutes and Proteobacteria represented approximately 57% of the microbiome. Approximately 70% of the detected thermophiles were strict anaerobes; however, Hydrogenobacter spp., obligate chemolithotrophic thermophiles, represented one of the major taxa. Several thermophilic photosynthetic microorganisms and acidothermophiles were also detected. Most of the phyla identified by 16S rRNA were also found using the shotgun metagenome approaches. The carbon, sulfur, and nitrogen metabolism within the SK hot spring community were evaluated by shotgun metagenome sequencing, and the data revealed diversity in terms of metabolic activity and dynamics. This hot spring has a rich diversified phylogenetic community partly due to its natural environment (plant litter, high TOC, and a shallow stream) and geochemical parameters (broad temperature and pH range). It is speculated that symbiotic relationships occur between the members of the community. Frontiers Research Foundation 2015 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/58324/1/ChiaSingChan2015_DiversityOfThermophilesInAMalaysianHotSpring.pdf Chan, Chia Sing and Chan, Kok Gan and Tay, Yea Ling and Chua, Yi Heng and Goh, Kian Mau (2015) Diversity of thermophiles in a Malaysian hot spring determined using 16S rRNA and shotgun metagenome sequencing. Frontiers in Microbiology, 6 . 177- 177. ISSN 1664-302X http://dx.doi.org/10.3389/fmicb.2015.00177 DOI:10.3389/fmicb.2015.00177 |
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Q Science (General) Chan, Chia Sing Chan, Kok Gan Tay, Yea Ling Chua, Yi Heng Goh, Kian Mau Diversity of thermophiles in a Malaysian hot spring determined using 16S rRNA and shotgun metagenome sequencing |
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The Sungai Klah (SK) hot spring is the second hottest geothermal spring in Malaysia. This hot spring is a shallow, 150-m-long, fast-flowing stream, with temperatures varying from 50 to 110°C and a pH range of 7.0-9.0. Hidden within a wooded area, the SK hot spring is continually fed by plant litter, resulting in a relatively high degree of total organic content (TOC). In this study, a sample taken from the middle of the stream was analyzed at the 16S rRNA V3-V4 region by amplicon metagenome sequencing. Over 35 phyla were detected by analyzing the 16S rRNA data. Firmicutes and Proteobacteria represented approximately 57% of the microbiome. Approximately 70% of the detected thermophiles were strict anaerobes; however, Hydrogenobacter spp., obligate chemolithotrophic thermophiles, represented one of the major taxa. Several thermophilic photosynthetic microorganisms and acidothermophiles were also detected. Most of the phyla identified by 16S rRNA were also found using the shotgun metagenome approaches. The carbon, sulfur, and nitrogen metabolism within the SK hot spring community were evaluated by shotgun metagenome sequencing, and the data revealed diversity in terms of metabolic activity and dynamics. This hot spring has a rich diversified phylogenetic community partly due to its natural environment (plant litter, high TOC, and a shallow stream) and geochemical parameters (broad temperature and pH range). It is speculated that symbiotic relationships occur between the members of the community. |
format |
Article |
author |
Chan, Chia Sing Chan, Kok Gan Tay, Yea Ling Chua, Yi Heng Goh, Kian Mau |
author_facet |
Chan, Chia Sing Chan, Kok Gan Tay, Yea Ling Chua, Yi Heng Goh, Kian Mau |
author_sort |
Chan, Chia Sing |
title |
Diversity of thermophiles in a Malaysian hot spring determined using 16S rRNA and shotgun metagenome sequencing |
title_short |
Diversity of thermophiles in a Malaysian hot spring determined using 16S rRNA and shotgun metagenome sequencing |
title_full |
Diversity of thermophiles in a Malaysian hot spring determined using 16S rRNA and shotgun metagenome sequencing |
title_fullStr |
Diversity of thermophiles in a Malaysian hot spring determined using 16S rRNA and shotgun metagenome sequencing |
title_full_unstemmed |
Diversity of thermophiles in a Malaysian hot spring determined using 16S rRNA and shotgun metagenome sequencing |
title_sort |
diversity of thermophiles in a malaysian hot spring determined using 16s rrna and shotgun metagenome sequencing |
publisher |
Frontiers Research Foundation |
publishDate |
2015 |
url |
http://eprints.utm.my/id/eprint/58324/1/ChiaSingChan2015_DiversityOfThermophilesInAMalaysianHotSpring.pdf http://eprints.utm.my/id/eprint/58324/ http://dx.doi.org/10.3389/fmicb.2015.00177 |
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