Adsorption of trypsin onto chitosan/Psf affinity membranes: Effects of physio-chemical environment
This study aimed to investigate the significance of the physico-chemical environment during trypsin adsorption onto a highly bio-specific affinity membrane. The affinity matrix polysulfone (PSf) membranes were prepared via a simple dry/wet phase inversion technique. Surface modification of PSf membr...
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my.utm.577082017-02-08T01:18:55Z http://eprints.utm.my/id/eprint/57708/ Adsorption of trypsin onto chitosan/Psf affinity membranes: Effects of physio-chemical environment Hamzah, Sofiah Ali, Nora’aini Mohd. Ariffin, Marinah Mohamad, Abdul Wahab Othman, Norasikin TP Chemical technology This study aimed to investigate the significance of the physico-chemical environment during trypsin adsorption onto a highly bio-specific affinity membrane. The affinity matrix polysulfone (PSf) membranes were prepared via a simple dry/wet phase inversion technique. Surface modification of PSf membranes was employed using chitosan in order to improve membrane hydrophilicity. Glutaraldehyde and ovomucoid were used as the membrane activator and affinity ligand, respectively. Inspection of membrane morphology was done using scanning electron microscopy. The functional groups on the membrane surface were determined using Fourier-transform infrared spectroscopy equipped with attenuated total reflection (ATR-FTIR). In order to determine the optimum conditions for the maximum adsorption capacity of trypsin, adsorption studies were performed at different pH levels (5, 7, 8, 10, 12), ionic strengths (0.01, 0.05, 0.1, 0.3 and 0.5 M) and initial trypsin concentrations (0.1, 0.3, 0.5, 0.7 and 0.9 mg/ml). The optimum adsorption was obtained with a 0.9 mg/ml initial trypsin solution at pH 7 and an ionic strength of 0.1 M Penerbit UTM Press 2015-05 Article PeerReviewed Hamzah, Sofiah and Ali, Nora’aini and Mohd. Ariffin, Marinah and Mohamad, Abdul Wahab and Othman, Norasikin (2015) Adsorption of trypsin onto chitosan/Psf affinity membranes: Effects of physio-chemical environment. Jurnal Teknologi, 74 (7). pp. 1-6. ISSN 0127-9696 http://dx.doi.org/10.11113/jt.v74.4686 DOI:10.11113/jt.v74.4686 |
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TP Chemical technology Hamzah, Sofiah Ali, Nora’aini Mohd. Ariffin, Marinah Mohamad, Abdul Wahab Othman, Norasikin Adsorption of trypsin onto chitosan/Psf affinity membranes: Effects of physio-chemical environment |
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This study aimed to investigate the significance of the physico-chemical environment during trypsin adsorption onto a highly bio-specific affinity membrane. The affinity matrix polysulfone (PSf) membranes were prepared via a simple dry/wet phase inversion technique. Surface modification of PSf membranes was employed using chitosan in order to improve membrane hydrophilicity. Glutaraldehyde and ovomucoid were used as the membrane activator and affinity ligand, respectively. Inspection of membrane morphology was done using scanning electron microscopy. The functional groups on the membrane surface were determined using Fourier-transform infrared spectroscopy equipped with attenuated total reflection (ATR-FTIR). In order to determine the optimum conditions for the maximum adsorption capacity of trypsin, adsorption studies were performed at different pH levels (5, 7, 8, 10, 12), ionic strengths (0.01, 0.05, 0.1, 0.3 and 0.5 M) and initial trypsin concentrations (0.1, 0.3, 0.5, 0.7 and 0.9 mg/ml). The optimum adsorption was obtained with a 0.9 mg/ml initial trypsin solution at pH 7 and an ionic strength of 0.1 M |
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Article |
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Hamzah, Sofiah Ali, Nora’aini Mohd. Ariffin, Marinah Mohamad, Abdul Wahab Othman, Norasikin |
author_facet |
Hamzah, Sofiah Ali, Nora’aini Mohd. Ariffin, Marinah Mohamad, Abdul Wahab Othman, Norasikin |
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Hamzah, Sofiah |
title |
Adsorption of trypsin onto chitosan/Psf affinity membranes: Effects of physio-chemical environment |
title_short |
Adsorption of trypsin onto chitosan/Psf affinity membranes: Effects of physio-chemical environment |
title_full |
Adsorption of trypsin onto chitosan/Psf affinity membranes: Effects of physio-chemical environment |
title_fullStr |
Adsorption of trypsin onto chitosan/Psf affinity membranes: Effects of physio-chemical environment |
title_full_unstemmed |
Adsorption of trypsin onto chitosan/Psf affinity membranes: Effects of physio-chemical environment |
title_sort |
adsorption of trypsin onto chitosan/psf affinity membranes: effects of physio-chemical environment |
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Penerbit UTM Press |
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2015 |
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http://eprints.utm.my/id/eprint/57708/ http://dx.doi.org/10.11113/jt.v74.4686 |
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