A study of the in-column detection performance for chromatography separation
This paper studies the in-column detection performance for chromatography separation. Numerical modeling was performed to investigate the detection efficiency of the electrodes that are suspended within a packed chromatography column. The results show that the density of the silica beads packed with...
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my.utm.576652017-02-01T01:27:58Z http://eprints.utm.my/id/eprint/57665/ A study of the in-column detection performance for chromatography separation Leow, Pei Ling Chee, Pei Song Patel, Bhavik Anil O’Hare, Danny TK Electrical engineering. Electronics Nuclear engineering This paper studies the in-column detection performance for chromatography separation. Numerical modeling was performed to investigate the detection efficiency of the electrodes that are suspended within a packed chromatography column. The results show that the density of the silica beads packed within the channel does not hinder the detection ability of the electrode. The findings have been further validated with experimental works. An 8 in-column electrode array provides the full separation progress traces of the five neurotransmitters and metabolites (adrenalin, dopamine, DOPAC, serotonin and 5-HIAA) within the column. From the in-column detection, better peak resolutions are developed though the separation column as compared to the conventional post-column detection. The whole progress of the separation shows that good separation (Rs > 1) can be obtained at E5 (28.7 mm from column inlet), whereas baseline separation could be obtained at E8 (last in-column electrode) with Rs ≥ 1.5 within 60 min. The presented results suggest that the packing materials within the column do not obstruct the efficiency of the electrodes but able to produce a good baseline separation detection as compared to conventional post-column detection. Springer Verlag 2015 Article PeerReviewed Leow, Pei Ling and Chee, Pei Song and Patel, Bhavik Anil and O’Hare, Danny (2015) A study of the in-column detection performance for chromatography separation. Microfluidics and Nanofluidics, 19 (2). pp. 343-349. ISSN 1613-4982 http://dx.doi.org/10.1007/s10404-015-1561-9 DOI:10.1007/s10404-015-1561-9 |
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TK Electrical engineering. Electronics Nuclear engineering Leow, Pei Ling Chee, Pei Song Patel, Bhavik Anil O’Hare, Danny A study of the in-column detection performance for chromatography separation |
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This paper studies the in-column detection performance for chromatography separation. Numerical modeling was performed to investigate the detection efficiency of the electrodes that are suspended within a packed chromatography column. The results show that the density of the silica beads packed within the channel does not hinder the detection ability of the electrode. The findings have been further validated with experimental works. An 8 in-column electrode array provides the full separation progress traces of the five neurotransmitters and metabolites (adrenalin, dopamine, DOPAC, serotonin and 5-HIAA) within the column. From the in-column detection, better peak resolutions are developed though the separation column as compared to the conventional post-column detection. The whole progress of the separation shows that good separation (Rs > 1) can be obtained at E5 (28.7 mm from column inlet), whereas baseline separation could be obtained at E8 (last in-column electrode) with Rs ≥ 1.5 within 60 min. The presented results suggest that the packing materials within the column do not obstruct the efficiency of the electrodes but able to produce a good baseline separation detection as compared to conventional post-column detection. |
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Article |
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Leow, Pei Ling Chee, Pei Song Patel, Bhavik Anil O’Hare, Danny |
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Leow, Pei Ling Chee, Pei Song Patel, Bhavik Anil O’Hare, Danny |
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Leow, Pei Ling |
title |
A study of the in-column detection performance for chromatography separation |
title_short |
A study of the in-column detection performance for chromatography separation |
title_full |
A study of the in-column detection performance for chromatography separation |
title_fullStr |
A study of the in-column detection performance for chromatography separation |
title_full_unstemmed |
A study of the in-column detection performance for chromatography separation |
title_sort |
study of the in-column detection performance for chromatography separation |
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Springer Verlag |
publishDate |
2015 |
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http://eprints.utm.my/id/eprint/57665/ http://dx.doi.org/10.1007/s10404-015-1561-9 |
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13.160551 |