Isolation and identification of 3-chloropropionic acid degrading bacterium from marine sponge

Synthetic haloalkanoic acids often applied as active components in herbicides are toxic to the environment and harmful to the living organisms. These compounds are widely released to the environment due to their routine use by agricultural activities. In recent years, accumulation of haloalkanoic ac...

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Main Authors: Mohd. Sufian, Nurul Hanani, Naim, Mohd. Azrul, Tengku Abdul Hamid, Tengku Haziyamin, Huyop, Fahrul, Abdul Hamid, Azzmer Azzar
Format: Article
Language:English
Published: Penerbit UTM Press 2015
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Online Access:http://eprints.utm.my/id/eprint/56072/1/FahrulZamanHuyop2015_IsolationandIdentificationof3-Chloropropionic.pdf
http://eprints.utm.my/id/eprint/56072/
http://dx.doi.org/10.11113/jt.v77.6742
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spelling my.utm.560722017-11-01T04:16:45Z http://eprints.utm.my/id/eprint/56072/ Isolation and identification of 3-chloropropionic acid degrading bacterium from marine sponge Mohd. Sufian, Nurul Hanani Naim, Mohd. Azrul Tengku Abdul Hamid, Tengku Haziyamin Huyop, Fahrul Abdul Hamid, Azzmer Azzar R Medicine (General) Synthetic haloalkanoic acids often applied as active components in herbicides are toxic to the environment and harmful to the living organisms. These compounds are widely released to the environment due to their routine use by agricultural activities. In recent years, accumulation of haloalkanoic acids was emerged in marine environment. In this study, a 3CP-degrading bacterium which designated as strain H4 was successfully isolated from marine sponge Gelliodes sp. that capable of degrading 3CP as the sole carbon and energy source. The bacteria growth on solid minimal media containing 3CP was the evident for the presence of dehalogenase enzyme. In liquid medium, the doubling time of the cells for strain H4 was 56.82 ± 0.1 h while the maximum chloride ion release was 2.03 ± 0.01 mM. The 16S rDNA sequence of strain H4 was obtained via 16s rRNA gene analysis (1000 bp) and it was closely related to Bacillus aryabhattai B8W22 (99% similarity). To the best of knowledge, this report is the first report detailing haloalkanoic acid degrading bacteria from marine sponge in coastal area of Malaysia Penerbit UTM Press 2015-09 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/56072/1/FahrulZamanHuyop2015_IsolationandIdentificationof3-Chloropropionic.pdf Mohd. Sufian, Nurul Hanani and Naim, Mohd. Azrul and Tengku Abdul Hamid, Tengku Haziyamin and Huyop, Fahrul and Abdul Hamid, Azzmer Azzar (2015) Isolation and identification of 3-chloropropionic acid degrading bacterium from marine sponge. Jurnal teknologi, 77 (25). pp. 71-75. ISSN 2180-3722 http://dx.doi.org/10.11113/jt.v77.6742 DOI:10.11113/jt.v77.6742
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic R Medicine (General)
spellingShingle R Medicine (General)
Mohd. Sufian, Nurul Hanani
Naim, Mohd. Azrul
Tengku Abdul Hamid, Tengku Haziyamin
Huyop, Fahrul
Abdul Hamid, Azzmer Azzar
Isolation and identification of 3-chloropropionic acid degrading bacterium from marine sponge
description Synthetic haloalkanoic acids often applied as active components in herbicides are toxic to the environment and harmful to the living organisms. These compounds are widely released to the environment due to their routine use by agricultural activities. In recent years, accumulation of haloalkanoic acids was emerged in marine environment. In this study, a 3CP-degrading bacterium which designated as strain H4 was successfully isolated from marine sponge Gelliodes sp. that capable of degrading 3CP as the sole carbon and energy source. The bacteria growth on solid minimal media containing 3CP was the evident for the presence of dehalogenase enzyme. In liquid medium, the doubling time of the cells for strain H4 was 56.82 ± 0.1 h while the maximum chloride ion release was 2.03 ± 0.01 mM. The 16S rDNA sequence of strain H4 was obtained via 16s rRNA gene analysis (1000 bp) and it was closely related to Bacillus aryabhattai B8W22 (99% similarity). To the best of knowledge, this report is the first report detailing haloalkanoic acid degrading bacteria from marine sponge in coastal area of Malaysia
format Article
author Mohd. Sufian, Nurul Hanani
Naim, Mohd. Azrul
Tengku Abdul Hamid, Tengku Haziyamin
Huyop, Fahrul
Abdul Hamid, Azzmer Azzar
author_facet Mohd. Sufian, Nurul Hanani
Naim, Mohd. Azrul
Tengku Abdul Hamid, Tengku Haziyamin
Huyop, Fahrul
Abdul Hamid, Azzmer Azzar
author_sort Mohd. Sufian, Nurul Hanani
title Isolation and identification of 3-chloropropionic acid degrading bacterium from marine sponge
title_short Isolation and identification of 3-chloropropionic acid degrading bacterium from marine sponge
title_full Isolation and identification of 3-chloropropionic acid degrading bacterium from marine sponge
title_fullStr Isolation and identification of 3-chloropropionic acid degrading bacterium from marine sponge
title_full_unstemmed Isolation and identification of 3-chloropropionic acid degrading bacterium from marine sponge
title_sort isolation and identification of 3-chloropropionic acid degrading bacterium from marine sponge
publisher Penerbit UTM Press
publishDate 2015
url http://eprints.utm.my/id/eprint/56072/1/FahrulZamanHuyop2015_IsolationandIdentificationof3-Chloropropionic.pdf
http://eprints.utm.my/id/eprint/56072/
http://dx.doi.org/10.11113/jt.v77.6742
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score 13.222552