γ-Fe2O3 nanoparticles filled polyvinyl alcohol as potential biomaterial for tissue engineering scaffold

Maghemite (γ-Fe2O3) nanoparticle with its unique magnetic properties is recently known to enhance the cell growth rate. In this study, γ-Fe2O3 is mixed into polyvinyl alcohol (PVA) matrix and then electrospun to form nanofibers. Design of experiments was used to determine the optimum parameter setti...

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Main Authors: Ngadiman, Nor Hasrul Akhmal, Idris, A., Irfan, Muhammad, Kurniawan, Denni, Mohd. Yusof, Noordin, Nasiri, Rozita
Format: Article
Language:English
Published: Elsevier BV 2015
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Online Access:http://eprints.utm.my/id/eprint/55774/1/NorHasrulNgadiman2015_NanoparticlesFilledPolyvinylAlcoholasPotentialBiomaterial.pdf
http://eprints.utm.my/id/eprint/55774/
http://dx.doi.org/10.1016/j.jmbbm.2015.04.029
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spelling my.utm.557742017-08-22T06:45:31Z http://eprints.utm.my/id/eprint/55774/ γ-Fe2O3 nanoparticles filled polyvinyl alcohol as potential biomaterial for tissue engineering scaffold Ngadiman, Nor Hasrul Akhmal Idris, A. Irfan, Muhammad Kurniawan, Denni Mohd. Yusof, Noordin Nasiri, Rozita TJ Mechanical engineering and machinery Maghemite (γ-Fe2O3) nanoparticle with its unique magnetic properties is recently known to enhance the cell growth rate. In this study, γ-Fe2O3 is mixed into polyvinyl alcohol (PVA) matrix and then electrospun to form nanofibers. Design of experiments was used to determine the optimum parameter settings for the electrospinning process so as to produce elctrospun mats with the preferred characteristics such as good morphology, Young's modulus and porosity. The input factors of the electrospinnning process were nanoparticles content (1-5%), voltage (25-35 kV), and flow rate (1-3 ml/h) while the responses considered were Young's modulus and porosity. Empirical models for both responses as a function of the input factors were developed and the optimum input factors setting were determined, and found to be at 5% nanoparticle content, 35 kV voltage, and 1 ml/h volume flow rate. The characteristics and performance of the optimum PVA/γ-Fe2O3 nanofiber mats were compared with those of neat PVA nanofiber mats in terms of morphology, thermal properties, and hydrophilicity. The PVA/γ-Fe2O3 nanofiber mats exhibited higher fiber diameter and surface roughness yet similar thermal properties and hydrophilicity compared to neat PVA PVA/γ-Fe2O3 nanofiber mats. Biocompatibility test by exposing the nanofiber mats with human blood cells was performed. In terms of clotting time, the PVA/γ-Fe2O3 nanofibers exhibited similar behavior with neat PVA. The PVA/γ-Fe2O3 nanofibers also showed higher cells proliferation rate when MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was done using human skin fibroblast cells. Thus, the PVA/γ-Fe2O3 electrospun nanofibers can be a promising biomaterial for tissue engineering scaffolds. Elsevier BV 2015-09-01 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/55774/1/NorHasrulNgadiman2015_NanoparticlesFilledPolyvinylAlcoholasPotentialBiomaterial.pdf Ngadiman, Nor Hasrul Akhmal and Idris, A. and Irfan, Muhammad and Kurniawan, Denni and Mohd. Yusof, Noordin and Nasiri, Rozita (2015) γ-Fe2O3 nanoparticles filled polyvinyl alcohol as potential biomaterial for tissue engineering scaffold. Journal of the Mechanical Behavior of Biomedical Materials, 49 . pp. 90-104. ISSN 1751-6161 http://dx.doi.org/10.1016/j.jmbbm.2015.04.029 DOI:10.1016/j.jmbbm.2015.04.029
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic TJ Mechanical engineering and machinery
spellingShingle TJ Mechanical engineering and machinery
Ngadiman, Nor Hasrul Akhmal
Idris, A.
Irfan, Muhammad
Kurniawan, Denni
Mohd. Yusof, Noordin
Nasiri, Rozita
γ-Fe2O3 nanoparticles filled polyvinyl alcohol as potential biomaterial for tissue engineering scaffold
description Maghemite (γ-Fe2O3) nanoparticle with its unique magnetic properties is recently known to enhance the cell growth rate. In this study, γ-Fe2O3 is mixed into polyvinyl alcohol (PVA) matrix and then electrospun to form nanofibers. Design of experiments was used to determine the optimum parameter settings for the electrospinning process so as to produce elctrospun mats with the preferred characteristics such as good morphology, Young's modulus and porosity. The input factors of the electrospinnning process were nanoparticles content (1-5%), voltage (25-35 kV), and flow rate (1-3 ml/h) while the responses considered were Young's modulus and porosity. Empirical models for both responses as a function of the input factors were developed and the optimum input factors setting were determined, and found to be at 5% nanoparticle content, 35 kV voltage, and 1 ml/h volume flow rate. The characteristics and performance of the optimum PVA/γ-Fe2O3 nanofiber mats were compared with those of neat PVA nanofiber mats in terms of morphology, thermal properties, and hydrophilicity. The PVA/γ-Fe2O3 nanofiber mats exhibited higher fiber diameter and surface roughness yet similar thermal properties and hydrophilicity compared to neat PVA PVA/γ-Fe2O3 nanofiber mats. Biocompatibility test by exposing the nanofiber mats with human blood cells was performed. In terms of clotting time, the PVA/γ-Fe2O3 nanofibers exhibited similar behavior with neat PVA. The PVA/γ-Fe2O3 nanofibers also showed higher cells proliferation rate when MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was done using human skin fibroblast cells. Thus, the PVA/γ-Fe2O3 electrospun nanofibers can be a promising biomaterial for tissue engineering scaffolds.
format Article
author Ngadiman, Nor Hasrul Akhmal
Idris, A.
Irfan, Muhammad
Kurniawan, Denni
Mohd. Yusof, Noordin
Nasiri, Rozita
author_facet Ngadiman, Nor Hasrul Akhmal
Idris, A.
Irfan, Muhammad
Kurniawan, Denni
Mohd. Yusof, Noordin
Nasiri, Rozita
author_sort Ngadiman, Nor Hasrul Akhmal
title γ-Fe2O3 nanoparticles filled polyvinyl alcohol as potential biomaterial for tissue engineering scaffold
title_short γ-Fe2O3 nanoparticles filled polyvinyl alcohol as potential biomaterial for tissue engineering scaffold
title_full γ-Fe2O3 nanoparticles filled polyvinyl alcohol as potential biomaterial for tissue engineering scaffold
title_fullStr γ-Fe2O3 nanoparticles filled polyvinyl alcohol as potential biomaterial for tissue engineering scaffold
title_full_unstemmed γ-Fe2O3 nanoparticles filled polyvinyl alcohol as potential biomaterial for tissue engineering scaffold
title_sort γ-fe2o3 nanoparticles filled polyvinyl alcohol as potential biomaterial for tissue engineering scaffold
publisher Elsevier BV
publishDate 2015
url http://eprints.utm.my/id/eprint/55774/1/NorHasrulNgadiman2015_NanoparticlesFilledPolyvinylAlcoholasPotentialBiomaterial.pdf
http://eprints.utm.my/id/eprint/55774/
http://dx.doi.org/10.1016/j.jmbbm.2015.04.029
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score 13.222552