Medium optimization for pullulanase production from anoxybacillus species using experimental design
The aim of the present study was to improve pullulanase production from the thermophilic Anoxybacillus species strain SK3-4 by medium engineering. The strain was isolated from a Malaysian hot spring, Sungai Klah (SK). The optimization processes were performed in two stages using 24 full factorial de...
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Online Access: | http://eprints.utm.my/id/eprint/53421/1/MadihahMdSalleh2014_MediumOptimizationforPullulanaseProductionfromAnoxybacillus.pdf http://eprints.utm.my/id/eprint/53421/ https://www.researchgate.net/publication/272238943_Medium_optimization_for_pullulanase_production_from_Anoxybacillus_species_using_experimental_design |
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my.utm.534212018-07-28T06:26:55Z http://eprints.utm.my/id/eprint/53421/ Medium optimization for pullulanase production from anoxybacillus species using experimental design Kahar, Ummirul Mukminin Md. Salleh, Madihah Goh, Kian Mau QH Natural history The aim of the present study was to improve pullulanase production from the thermophilic Anoxybacillus species strain SK3-4 by medium engineering. The strain was isolated from a Malaysian hot spring, Sungai Klah (SK). The optimization processes were performed in two stages using 24 full factorial design and central composite rotatable design (CCRD). Four variables were manipulated to improve pullulanase yield: initial culture pH, and concentrations of pullulan, tryptone and ammonium chloride by using a 24 full factorial design. Following that a quadratic model of CCRD was then used to identify the optimum yield of pullulanase by manipulating all four of these significant parameters. The optimized medium composition was a pH of 8.29, 0.79% (w/v) pullulan, 0.12% (w/v) tryptone and 0.39% (w/v) ammonium chloride, giving a final pullulanase activity of 3.86 U/mL and specific activity of 3.38 U/mg. The specific activity achieved was higher than the CCRD predicted value (3.00 U/mg). The application of two different experimental designs resulted in an improvement of pullulanase specific activity of approximately 73% from the original Thermus medium National Institute of Science Communication and Information Resources (NISCAIR) 2014 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/53421/1/MadihahMdSalleh2014_MediumOptimizationforPullulanaseProductionfromAnoxybacillus.pdf Kahar, Ummirul Mukminin and Md. Salleh, Madihah and Goh, Kian Mau (2014) Medium optimization for pullulanase production from anoxybacillus species using experimental design. Indian Journal of Biotechnology, 13 (1). pp. 89-97. ISSN 0975-0967 https://www.researchgate.net/publication/272238943_Medium_optimization_for_pullulanase_production_from_Anoxybacillus_species_using_experimental_design |
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QH Natural history Kahar, Ummirul Mukminin Md. Salleh, Madihah Goh, Kian Mau Medium optimization for pullulanase production from anoxybacillus species using experimental design |
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The aim of the present study was to improve pullulanase production from the thermophilic Anoxybacillus species strain SK3-4 by medium engineering. The strain was isolated from a Malaysian hot spring, Sungai Klah (SK). The optimization processes were performed in two stages using 24 full factorial design and central composite rotatable design (CCRD). Four variables were manipulated to improve pullulanase yield: initial culture pH, and concentrations of pullulan, tryptone and ammonium chloride by using a 24 full factorial design. Following that a quadratic model of CCRD was then used to identify the optimum yield of pullulanase by manipulating all four of these significant parameters. The optimized medium composition was a pH of 8.29, 0.79% (w/v) pullulan, 0.12% (w/v) tryptone and 0.39% (w/v) ammonium chloride, giving a final pullulanase activity of 3.86 U/mL and specific activity of 3.38 U/mg. The specific activity achieved was higher than the CCRD predicted value (3.00 U/mg). The application of two different experimental designs resulted in an improvement of pullulanase specific activity of approximately 73% from the original Thermus medium |
format |
Article |
author |
Kahar, Ummirul Mukminin Md. Salleh, Madihah Goh, Kian Mau |
author_facet |
Kahar, Ummirul Mukminin Md. Salleh, Madihah Goh, Kian Mau |
author_sort |
Kahar, Ummirul Mukminin |
title |
Medium optimization for pullulanase production from anoxybacillus species using experimental design |
title_short |
Medium optimization for pullulanase production from anoxybacillus species using experimental design |
title_full |
Medium optimization for pullulanase production from anoxybacillus species using experimental design |
title_fullStr |
Medium optimization for pullulanase production from anoxybacillus species using experimental design |
title_full_unstemmed |
Medium optimization for pullulanase production from anoxybacillus species using experimental design |
title_sort |
medium optimization for pullulanase production from anoxybacillus species using experimental design |
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National Institute of Science Communication and Information Resources (NISCAIR) |
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2014 |
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http://eprints.utm.my/id/eprint/53421/1/MadihahMdSalleh2014_MediumOptimizationforPullulanaseProductionfromAnoxybacillus.pdf http://eprints.utm.my/id/eprint/53421/ https://www.researchgate.net/publication/272238943_Medium_optimization_for_pullulanase_production_from_Anoxybacillus_species_using_experimental_design |
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